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Related Experiment Video

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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

MPIC: a high-throughput analytical method for multiple DNA targets.

Jinchao Guo¹, Litao Yang, Lili Chen

¹GMO Detection Laboratory, SJTU-Bor Luh Food Safety Center, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, People's Republic of China.

Analytical Chemistry

|February 5, 2011

Summary

This summary is machine-generated.

A new multiplex Microdroplet PCR Implemented Capillary gel electrophoresis (MPIC) method enables high-throughput DNA target analysis. This novel approach allows simultaneous detection of 24 targets for applications like GMO testing.

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Last Updated: Jun 4, 2026

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High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

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Published on: August 3, 2011

Area of Science:

Molecular Biology
Biotechnology
Analytical Chemistry

Background:

High-throughput analysis of multiple DNA targets is crucial for various applications, including diagnostics and quality control.
Existing methods often face limitations in throughput, sensitivity, or multiplexing capability.

Purpose of the Study:

To develop and validate a novel, high-throughput method for simultaneous analysis of multiple DNA targets.
To demonstrate the utility of the developed method in genetically modified organism (GMO) detection.

Main Methods:

Development of a two-step PCR amplification strategy: multiplex Microdroplet PCR Implemented Capillary gel electrophoresis (MPIC).
First step involves preamplification using bipartite primers with universal tails.
Second step utilizes microdroplet compartmentalization for parallel amplification and subsequent capillary gel electrophoresis (CGE) analysis.

Main Results:

Simultaneous detection of 24 DNA targets was achieved using the MPIC system.
A relative limit of detection of 0.1% (w/w) and an absolute limit of detection of 39 target DNA copies were established.
The method demonstrated high throughput and sensitivity in GMO analysis.

Conclusions:

The MPIC system offers a promising and efficient alternative for high-throughput analysis of multiple DNA targets.
This novel approach significantly enhances capabilities in complex DNA target detection and quantification.
The MPIC method holds potential for broad applications in molecular diagnostics, food safety, and genetic research.