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The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo
Published on: October 30, 2014
Development of the olfactory system in turbot (Psetta maxima L.)
M J Doldán1, P Cid, L Mantilla
1Laboratory of Cell Biology, Department of Functional Biology, University of Vigo, 36200 Vigo, Spain.
Journal of Chemical Neuroanatomy
|February 5, 2011
Summary
This study details turbot olfactory system development, revealing how olfactory receptor cells differentiate and sensory neurons reach the brain. Proliferative activity in the olfactory bulb shifts during larval and metamorphic stages.
Area of Science:
- Developmental Biology
- Neuroscience
- Ichthyology
Background:
- The olfactory system's development is crucial for fish survival and behavior.
- Understanding turbot olfactory histogenesis provides insights into teleost sensory system evolution.
Purpose of the Study:
- To investigate the developmental process of the turbot olfactory system.
- To map the spatio-temporal distribution of cell proliferation and neuronal differentiation.
Main Methods:
- Histological analysis of turbot embryos and larvae.
- Immunohistochemistry using Proliferating Cell Nuclear Antigen (PCNA) and Calretinin (CR).
Main Results:
- Olfactory placodes transform into pits, with the right organ migrating during metamorphosis.
- Ciliated cells differentiate before microvillous cells; crypt cells form during metamorphosis.
- Axons reach the forebrain by hatching; CR-ir glomerular fields appear during larval stages.
- Olfactory bulb organization changes, becoming distorted with asymmetric glomeruli during metamorphosis.
- Proliferative activity in the olfactory bulb is concentrated in the ventral periventricular zone.
Conclusions:
- Turbot olfactory development follows patterns seen in other teleosts, with specific timing for cell differentiation.
- Metamorphosis significantly alters olfactory organ structure and olfactory bulb organization.
- Cell proliferation dynamics in the olfactory bulb change throughout turbot development.
