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Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
Isolation and phylogenetic analysis of Batai virus, Germany
Hanna Jöst1, Alexandra Bialonski, Christel Schmetz
1German Mosquito Control Association, Waldsee, Germany. hanna.joest@gmx.de
The American Journal of Tropical Medicine and Hygiene
|February 5, 2011
Summary
Batai virus (BATV) was detected in mosquitoes in Germany for the first time. Molecular analysis confirmed the presence of BATV, showing its relation to European strains.
Area of Science:
- Virology
- Entomology
- Molecular Biology
Background:
- Batai virus (BATV) is an arbovirus with a known presence in parts of Europe.
- Previous studies had not documented BATV circulation within Germany.
Purpose of the Study:
- To investigate the presence and genetic characteristics of Batai virus in mosquitoes in Southwest Germany.
- To determine the genetic relationship of any detected BATV strains to other known strains.
Main Methods:
- A molecular survey was conducted, involving the collection and analysis of 16,057 mosquitoes in Southwest Germany during the summer of 2009.
- Partial S, M, and L viral RNA segments were sequenced and analyzed.
- Phylogenetic analysis was performed on the obtained sequences.
Main Results:
- Batai virus (BATV) was identified in mosquitoes belonging to the Anopheles maculipennis sensu lato complex.
- Sequence analysis of partial S, M, and L segments confirmed the presence of BATV and indicated no evidence of viral reassortment.
- Phylogenetic analysis revealed that the German BATV strain is closely related to strains found in Slovakia, Ukraine, and Russia.
Conclusions:
- This study provides the first molecular evidence of Batai virus circulation in Germany.
- The identified German BATV strain shares close genetic links with other European strains, suggesting established viral lineages.
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