Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Stem Cell Culture01:17

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Potential Factors Influencing Heart Rates During Locomotor Training Poststroke and Their Associations With Locomotor Outcomes.

Journal of neurologic physical therapy : JNPT·2026
Same author

Central sensitization: when is this the right terminology?

Pain·2026
Same author

The Concept of Shaping Applied to Locomotor Interventions: Clinical and Robotic Strategies to Facilitate and Progress Variable Stepping Training at Higher Intensities.

Neurorehabilitation and neural repair·2026
Same author

Correction: Bortezomib-induced neurotoxicity in human neurons is the consequence of nicotinamide adenine dinucleotide depletion.

Disease models & mechanisms·2026
Same author

A human iPSC-derived sensory neuron platform for high-throughput discovery of neuroprotectants against chemotherapy-induced peripheral neuropathy.

Cell reports. Medicine·2026
Same author

Targeting G <sub>i/o</sub> -coupled GPCRs to inhibit nociceptors: insights from the serotonin receptor Htr1b and triptans.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jun 4, 2026

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
12:03

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Published on: October 31, 2012

A functionally characterized test set of human induced pluripotent stem cells.

Gabriella L Boulting1, Evangelos Kiskinis, Gist F Croft

  • 1The Howard Hughes Medical Institute, Cambridge, Massachusetts, USA.

Nature Biotechnology
|February 5, 2011
PubMed
Summary

Human induced pluripotent stem cells (iPSCs) are valuable for research, showing consistent differentiation into motor neurons despite variability. This study confirms their utility for stem cell biology and disease modeling.

More Related Videos

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
10:48

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

Published on: July 7, 2017

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
09:02

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Published on: June 9, 2017

Related Experiment Videos

Last Updated: Jun 4, 2026

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector
12:03

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Published on: October 31, 2012

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix
10:48

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

Published on: July 7, 2017

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing
09:02

Protocol for the Differentiation of Human Induced Pluripotent Stem Cells into Mixed Cultures of Neurons and Glia for Neurotoxicity Testing

Published on: June 9, 2017

Area of Science:

  • Stem Cell Biology
  • Developmental Neuroscience
  • Regenerative Medicine

Background:

  • Human induced pluripotent stem cells (iPSCs) offer potential for development studies and in vitro disease modeling.
  • Variability in iPSC behavior has raised concerns about their reliability and utility in research.

Purpose of the Study:

  • To assess the differentiation capacity of a diverse set of human induced pluripotent stem cell (iPSC) lines.
  • To determine if iPSCs can reliably differentiate into functional motor neurons under standardized conditions.
  • To establish a robust iPSC and human embryonic stem cell (ESC) test set for research applications.

Main Methods:

  • Characterization of 16 iPSC lines from seven individuals for pluripotency and differentiation potential.
  • Standardized motor neuron differentiation protocols applied in two independent laboratories.
  • Assessment of differentiation efficiency and rescue of neural differentiation in resistant lines.

Main Results:

  • Thirteen of 16 iPSC lines successfully generated functional motor neurons, comparable to human embryonic stem cells (ESCs).
  • Three iPSC lines initially resistant to neural differentiation were rescued by early neuralization protocols.
  • All tested iPSC lines demonstrated differentiation capacity, irrespective of karyotype or marker expression variations.

Conclusions:

  • Human induced pluripotent stem cells (iPSCs) exhibit reliable differentiation capacity for motor neuron generation, validating their use in research.
  • Standardized protocols and early neuralization can overcome initial differentiation challenges in some iPSC lines.
  • The established iPSC and ESC test set serves as a valuable resource for stem cell biology and disease modeling research.