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Synthesis of fluorescent probes based on the pyochelin siderophore scaffold

Sabrina Noël1, Laurent Guillon, Isabelle J Schalk

  • 1UMR 7242 Biotechnologie et Signalisation Cellulaire, Université de Strasbourg-CNRS, ESBS, Illkirch, France.

Organic Letters
|February 8, 2011
PubMed

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...