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Related Experiment Video

Updated: Jun 4, 2026

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance
07:32

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

Published on: December 3, 2020

Epithelial barriers in intestinal inflammation.

Lena J John1, Michael Fromm, Jörg-Dieter Schulzke

  • 1Department of General Medicine, Charité, Campus Benjamin Franklin, Berlin, Germany.

Antioxidants & Redox Signaling
|February 8, 2011
PubMed
Summary
This summary is machine-generated.

Inflammation disrupts the intestinal barrier, increasing permeability. Oxidative stress, a key factor in inflammation, further impairs this barrier, particularly affecting epithelial tight junctions.

Related Experiment Videos

Last Updated: Jun 4, 2026

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance
07:32

An Intravital Microscopy-Based Approach to Assess Intestinal Permeability and Epithelial Cell Shedding Performance

Published on: December 3, 2020

Area of Science:

  • Gastroenterology
  • Cell Biology
  • Immunology

Background:

  • The gastrointestinal epithelium acts as a selective barrier, regulating transport and preventing pathogen entry.
  • Inflammation can compromise this barrier, leading to increased permeability and reduced electrical resistance.
  • Oxidative stress is a significant factor associated with inflammatory processes in the gut.

Purpose of the Study:

  • To review the impact of inflammatory oxidative stress on intestinal barrier function.
  • To highlight the role of epithelial tight junctions in maintaining barrier integrity during inflammation.
  • To discuss relevant diseases, cytokines, and therapeutic strategies.

Main Methods:

  • Review of existing literature on intestinal barrier function, inflammation, and oxidative stress.
  • Analysis of the role of tight junctions in disease pathogenesis.
  • Discussion of cytokines involved in oxidative stress and barrier disruption.

Main Results:

  • Inflammatory oxidative stress significantly impairs epithelial barrier function.
  • Tight junctions are key targets affected by oxidative stress during inflammation.
  • Several gastrointestinal diseases, including inflammatory bowel diseases and celiac disease, exhibit barrier dysfunction.

Conclusions:

  • Oxidative stress is a critical mediator of intestinal barrier disruption during inflammation.
  • Understanding the mechanisms of oxidative stress on tight junctions is crucial for therapeutic development.
  • Targeting oxidative stress pathways may offer novel therapeutic strategies for gastrointestinal barrier disorders.