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Published on: February 11, 2019
Stabilizing effect of polyols is sensitive to inherent stability of protein
Md Zahid Kamal1, Shoeb Ahmad, Nalam Madhusudhana Rao
1Centre for Cellular and Molecular Biology, Hyderabad, India. zahid@ccmb.res.in
Abstract:
In studies on polyol-mediated protein stabilization, the polyols are the preferred variable and less importance is given to the intrinsic properties of the protein used. We investigated the stabilizing effects of glycerol on three in vitro evolved lipase mutants with varying stabilities and also in a broad pH range of 3.3-12.1. Significant linear negative correlation between increment in stability due to glycerol and prior stability suggests that stabilizing effects of glycerol depend on the prior stability of the protein. Polar/nonpolar surface area and charge do not have a bearing on the stabilizing effects of glycerol.
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