Related Experiment Video
Updated: Jun 4, 2026

Generation of Enterobacter sp. YSU Auxotrophs Using Transposon Mutagenesis
Published on: October 31, 2014
Engineering the robustness of Clostridium acetobutylicum by introducing glutathione biosynthetic capability
Linjiang Zhu1, Hongjun Dong, Yanping Zhang
1Institute of Microbiology, Chinese Academy of Sciences, Chaoyang District, Beijing, China
Abstract:
To improve the aero- and solvent tolerance of the solvent-producing Clostridium acetobutylicum, glutathione biosynthetic capability was introduced into C. acetobutylicum DSM1731 by cloning and over-expressing the gshAB genes from Escherichia coli. Strain DSM1731(pITAB) produces glutathione, and shows a significantly improved survival upon aeration and butanol challenge, as compared with the control. In addition, strain DSM1731(pITAB) exhibited an improved butanol tolerance and an increased butanol production capability, as compared with the recombinant strains with only gshA or gshB gene. These results illustrated that introducing glutathione biosynthetic pathway, which is redundant for the metabolism of C. acetobutylicum, can increase the robustness of the host to achieve a better solvent production.
More Related Videos
Related Concept Videos
Bioreactor Controls-III
Production of Pharmaceuticals
Production of Antibiotics
Biosynthesis in Bacteria

