Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bacterial Transformation

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...

Bacterial Transformation

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Removal of antibiotics metronidazole, sulfamethoxazole, and trimethoprim by the green microalga Chlorella sorokiniana, and assessment of their biotransformation products.

The Science of the total environment·2026

Same author

Flagellar glycosylation with pseudaminic acids is widespread in the genus Clostridium.

BMC microbiology·2026

Same author

Reproductive hormone stability with prolonged intranasal oxytocin in adults with obesity.

International journal of obesity (2005)·2026

Same author

Assembly of a homohexameric minichromosome maintenance complex is dependent on ATP and DNA.

The Journal of biological chemistry·2025

Same author

Oxytocin levels do not change around a meal and correlate with reward-driven caloric consumption in adults with obesity.

Diabetes, obesity & metabolism·2025

Same author

Study of the green microalga Chlorella sorokiniana for the removal of nutrients and multiple antibiotics in wastewater treatment.

Environmental toxicology and chemistry·2025

Same journal

A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.

Applied and environmental microbiology·2026

Same journal

Sources and traits of bacteria and fungi found in the near-surface atmosphere.

Applied and environmental microbiology·2026

Same journal

Advancing One Health in Africa through continental early warning environmental surveillance.

Applied and environmental microbiology·2026

Same journal

Specificity and longevity of a bacterial interspecies mutual cooperation benefiting organic micropollutant biodegradation.

Applied and environmental microbiology·2026

Same journal

Coordinated regulation of trimethylamine catabolism in abundant marine bacteria.

Applied and environmental microbiology·2026

Same journal

Synergistic and individual effects of RNase E, II, and R in the regulation of <i>Escherichia coli</i> growth and metabolism.

Applied and environmental microbiology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

CRISPR-based Shuttle Cloning: A High-throughput Cloning Method

CRISPR-based Shuttle Cloning: A High-throughput Cloning Method

Published on: June 13, 2025

Shuttle vector system for Methanococcus maripaludis with improved transformation efficiency.

Alison D Walters¹, Sarah E Smith, James P J Chong

¹Department of Biology (Area 5), University of York, Wentworth Way, York YO10 5DD, United Kingdom.

Applied and Environmental Microbiology

|February 8, 2011

Summary

This summary is machine-generated.

Researchers found a specific DNA element that helps shuttle vectors function in Methanococcus maripaludis. This discovery significantly improves transformation efficiency for related vectors in this microorganism.

More Related Videos

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Published on: December 8, 2023

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Published on: February 2, 2018

Related Experiment Videos

Last Updated: Jun 4, 2026

CRISPR-based Shuttle Cloning: A High-throughput Cloning Method

CRISPR-based Shuttle Cloning: A High-throughput Cloning Method

Published on: June 13, 2025

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Published on: December 8, 2023

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection

Published on: February 2, 2018

Area of Science:

Microbiology
Molecular Biology
Genetics

Background:

Shuttle vectors are crucial tools for genetic manipulation in microorganisms.
Methanococcus maripaludis is an important archaeon with limited genetic tools.
Efficient maintenance and transformation of vectors are essential for studying M. maripaludis.

Purpose of the Study:

To identify genetic elements sufficient for shuttle vector maintenance in Methanococcus maripaludis.
To enhance the transformation efficiency of shuttle vectors in M. maripaludis.

Main Methods:

Identification and characterization of an open reading frame (ORF1) and a DNA element from the pURB500 vector.
Integration of ORF1 into the M. maripaludis genome (Strain S0001).
Transformation efficiency assays using pURB500-based vectors and a smaller shuttle vector (pAW42).

Main Results:

An open reading frame (ORF1) and an associated DNA element were identified as sufficient for shuttle vector maintenance.
Strain S0001, engineered to contain ORF1, supported a significantly smaller shuttle vector (pAW42).
A 7,000-fold increase in transformation efficiency was observed for pURB500-based vectors in the engineered strain.

Conclusions:

The identified ORF1 and DNA element are key for shuttle vector replication and maintenance in M. maripaludis.
This finding provides a novel system for enhanced genetic manipulation of M. maripaludis.
The improved transformation efficiency opens new avenues for functional genomics in this archaeon.