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Related Concept Videos

Cell Migration01:09

Cell Migration

Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.

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Related Experiment Video

Updated: Jun 4, 2026

A Cost Effective and Adaptable Scratch Migration Assay
08:59

A Cost Effective and Adaptable Scratch Migration Assay

Published on: June 30, 2020

A quantitative, facile, and high-throughput image-based cell migration method is a robust alternative to the scratch

Wendy Gough1, Keren I Hulkower, Renee Lynch

  • 1Department of Quantitative and Structural Biology, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, IN 46285, USA. gough_wendy@lilly.com

Journal of Biomolecular Screening
|February 8, 2011
PubMed
Summary

The Oris™ Cell Migration Assay offers a more reproducible and quantitative alternative to the scratch assay for evaluating cell migration. This method, combined with the Acumen™ Explorer, provides robust and cost-effective cell migration analysis.

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Last Updated: Jun 4, 2026

A Cost Effective and Adaptable Scratch Migration Assay
08:59

A Cost Effective and Adaptable Scratch Migration Assay

Published on: June 30, 2020

Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera
05:27

Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera

Published on: August 8, 2018

A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay
11:30

A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay

Published on: December 4, 2012

Area of Science:

  • Cell biology
  • Biotechnology
  • Drug discovery

Background:

  • Cell migration is crucial for therapeutic responses like angiogenesis.
  • The scratch assay is a common but often inconsistent method for assessing cell migration.
  • Quantitative structure-activity relationship studies require highly reproducible migration assays.

Purpose of the Study:

  • To compare the Oris™ Cell Migration Assay with the traditional scratch assay.
  • To evaluate the efficacy of the Oris™ assay for quantitative cell migration analysis.
  • To assess the use of the Acumen™ Explorer laser microplate cytometer with the Oris™ assay.

Main Methods:

  • Comparison of scratch assay and Oris™ Cell Migration Assay.
  • Utilized an endothelial progenitor cell line.
  • Tested the Src kinase inhibitor dasatinib.
  • Employed the Acumen™ Explorer laser microplate cytometer.

Main Results:

  • The Oris™ assay allows for precisely placed and uniformly sized cell-free areas.
  • The Oris™ assay avoids issues associated with the scratch assay, such as factor release from damaged cells.
  • The combination of Oris™ assay and Acumen™ Explorer demonstrated robust, efficient, and cost-effective cell migration analysis with excellent metrics.

Conclusions:

  • The Oris™ Cell Migration Assay is a superior alternative to the scratch assay for quantitative cell migration studies.
  • The Acumen™ Explorer laser microplate cytometer enhances the Oris™ assay's performance.
  • This combined system offers a validated, reproducible, and cost-effective solution for cell migration assessment.