Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 4, 2026

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
06:53

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

High-throughput single-molecule optofluidic analysis.

Soohong Kim1, Aaron M Streets, Ron R Lin

  • 1Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.

Nature Methods
|February 8, 2011
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epigenetic conditioning improves sequence-based modeling of gene regulation across cell types and alleles.

bioRxiv : the preprint server for biology·2026
Same author

Tunable electrostatic interactions of lipid-coated quantum dots with biological membranes.

bioRxiv : the preprint server for biology·2026
Same author

SDXL model-based optimization for interior design: Data-driven and deep learning methods.

PloS one·2026
Same author

Scattering-based super-resolution optical fluctuation imaging.

Optics express·2025
Same author

Toward measurements of absolute membrane potential in Bacillus subtilis using fluorescence lifetime.

Biophysical reports·2025
Same author

On-ratio PDMS bonding for multilayer microfluidic device fabrication.

Journal of micromechanics and microengineering : structures, devices, and systems·2024
Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026
Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026
Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026
Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026
Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026
See all related articles

We developed an automated single-molecule measurement system using microfluidics for precise biomolecule titration. This system rapidly analyzes molecular behavior and enzyme activity, like transcription, under varying chemical conditions.

Area of Science:

  • Biophysics
  • Biochemistry
  • Molecular Biology

Background:

  • Single-molecule measurements are crucial for understanding molecular mechanisms.
  • High-throughput and automated systems are needed to accelerate research.
  • Microfluidics offers precise control over small volumes for biochemical assays.

Purpose of the Study:

  • To present a novel high-throughput, automated single-molecule measurement system.
  • To demonstrate the system's capability for precise biomolecule titration.
  • To showcase its application in studying molecular conformation and enzymatic activity.

Main Methods:

  • Development of an automated single-molecule measurement platform.
  • Integration of a microfluidic mixing device with valves and pumps.

More Related Videos

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

Related Experiment Videos

Last Updated: Jun 4, 2026

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies
06:53

Use of Dual Optical Tweezers and Microfluidics for Single-Molecule Studies

Published on: November 18, 2022

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
10:57

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Published on: November 2, 2009

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)
07:19

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM)

Published on: June 28, 2017

  • Picoliter-resolution titration of biomolecules.
  • Monitoring of transcription by Escherichia coli RNA polymerase.

    • Main Results:

    • The system enables high-throughput, automated single-molecule analysis.
    • Precise biomolecule titration with picoliter resolution was achieved.
    • Rapid sampling of biomolecule conformational landscape was demonstrated.
    • Enzymatic activity, specifically transcription, was measured as a function of the chemical environment.

    Conclusions:

    • The described system provides a powerful tool for biochemical and biophysical research.
    • It facilitates rapid investigation of molecular dynamics and enzyme kinetics.
    • The microfluidic approach allows for detailed analysis of molecular behavior under controlled conditions.