Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Relationship between thyroid autoantibody spectrotype and IgG subclass.

N Fukuma1, S M McLachlan, V B Petersen

  • 1Department of Medicine, University of Wales College of Medicine, Cardiff, UK.

Autoimmunity
|January 1, 1990
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Inhibition of HERV-K (HML-2) in amyotrophic lateral sclerosis patients on antiretroviral therapy.

Journal of the neurological sciences·2021
Same author

Detection of first gammaherpesvirus sequences in Central African bats.

New microbes and new infections·2020
Same author

Viral emergence in marine mammals in the North Pacific may be linked to Arctic sea ice reduction.

Scientific reports·2019
Same author

Effects of difructose anhydride III on serum immunoglobulin G concentration and health status of newborn Holstein calves during the preweaning period.

Journal of dairy science·2018
Same author

A Health Services Research Agenda for Bariatric Surgery Within the Veterans Health Administration.

Journal of general internal medicine·2017
Same author

Genetic dissection of intraspecific variation in a male-specific sexual trait in Drosophila melanogaster.

Heredity·2016
Same journal

Platycodin D attenuates proliferation, migration, and angiogenesis in RA-stimulated endothelial cells via CD146 modulation.

Autoimmunity·2026
Same journal

CCDC77 and SLC45A3 mediate the genetic mechanism of Hashimoto's thyroiditis through IL-6.

Autoimmunity·2026
Same journal

Multi-cohort transcriptomic analysis with machine learning identifies interferon-related candidate genes in systemic lupus erythematosus.

Autoimmunity·2026
Same journal

IgA Vasculitis with necrotizing arteritis: a multicenter retrospective study from the French Vasculitis Study Group and systematic review of the literature.

Autoimmunity·2026
Same journal

Global research trends in ferroptosis in autoimmune diseases: a bibliometric and visual analysis (2018-2025).

Autoimmunity·2026
Same journal

Monocyte exhaustion associated with systemic lupus erythematosus.

Autoimmunity·2026
See all related articles

Thyroid autoantibody spectrotypes, reflecting IgG subclass fingerprints, are linked to Graves' and Hashimoto's disease. These patterns may indicate an individual's immune response to thyroglobulin (Tg) and thyroid peroxidase (TPO) antigens.

Area of Science:

  • Immunology
  • Endocrinology
  • Autoimmunity

Background:

  • Autoantibodies to thyroglobulin (Tg) and thyroid peroxidase (TPO) are key biomarkers in autoimmune thyroid diseases like Hashimoto's and Graves'.
  • The heterogeneity of autoantibody responses, particularly their spectrotypes and IgG subclass distribution, is not fully understood in relation to disease pathogenesis.

Purpose of the Study:

  • To investigate the relationship between the spectrotype (isoelectric point distribution) and IgG subclass of autoantibodies against Tg and TPO.
  • To determine if spectrotype patterns correlate with specific IgG subclasses and potentially reflect immune response characteristics.

Main Methods:

  • Sera from patients with Hashimoto's and Graves' disease were analyzed.
  • Isoelectric focusing (IEF) was performed on autoantibodies, followed by transblotting to nitrocellulose.

Related Experiment Videos

  • Filters were probed with radiolabeled Tg and TPO to identify antibody spectrotypes and their corresponding IgG subclasses.

    • Main Results:

    • Thyroid peroxidase (TPO) autoantibody spectrotypes showed patient-specific patterns that remained consistent over time (2-5 years).
    • A correlation was observed between spectrotype and IgG subclass; for example, IgG1 Tg autoantibodies focused at higher pI values (more cathodic) than IgG4 Tg autoantibodies.
    • Antibodies of multiple subclasses (IgG1+2+4) exhibited broad spectrotype profiles across a wide pI range.

    Conclusions:

    • The spectrotypes of Tg and TPO autoantibodies appear to be characteristic "fingerprints" determined by their IgG subclass composition.
    • These IgG subclass "fingerprints" may serve as a measure of an individual's immune system's ability to recognize diverse epitopes on Tg and TPO antigens.
    • Understanding these spectrotype-subclass relationships offers insights into the specific immune responses in autoimmune thyroid diseases.