Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 4, 2026

Isolation and Identification of Limbal Niche Cells
10:11

Isolation and Identification of Limbal Niche Cells

Published on: October 27, 2023

Human epithelial cell cultures from superficial limbal explants.

D Ghoubay-Benallaoua1, E Basli, P Goldschmidt

  • 1Institut de la Vision, UPMC Univ Paris 06, UMR_S 968 / INSERM, U968 / CHNO des XV-XX / CNRS, UMR_7210, Paris, France. benallaouadjida@yahoo.fr

Molecular Vision
|February 8, 2011
PubMed
Summary

Superficial limbal explants yield better human limbal epithelial cell cultures than full-thickness explants. Surgical preparation enhances culture success, growth, and progenitor cell preservation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epidemiological changes in cutaneous lymphomas: an analysis of 8593 patients from the French Cutaneous Lymphoma Registry.

The British journal of dermatology·2020
Same author

Boosting the sterile insect technique with pyriproxyfen increases tsetse flies Glossina palpalis gambiensis sterilization in controlled conditions.

Scientific reports·2020
Same author

Atypical severe diffuse lamellar keratitis presenting as concentric rings after femtosecond laser-assisted small-incision lenticule extraction (SMILE).

Journal francais d'ophtalmologie·2020
Same author

Sterile keratitis following standard corneal collagen crosslinking: A case series and literature review.

Journal francais d'ophtalmologie·2019
Same author

[In vivo confocal microscopy and optical coherence tomography in stromal corneal dystrophies].

Journal francais d'ophtalmologie·2019
Same author

[Imaging of Reis-Bückler corneal dystrophy].

Journal francais d'ophtalmologie·2018

Area of Science:

  • Ophthalmology
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • The limbus is crucial for corneal epithelial maintenance and regeneration.
  • Culturing limbal epithelial cells is vital for treating ocular surface diseases.
  • Optimizing culture conditions is essential for clinical applications.

Purpose of the Study:

  • To evaluate the growth kinetics and cell phenotype of human limbal explants in a feeder-free, cholera toxin-free medium.
  • To compare the efficacy of superficial versus full-thickness limbal explants for cell culture.
  • To assess the impact of surgical preparation on limbal explant culture outcomes.

Main Methods:

  • Human organ-cultured corneas were used to generate superficial and full-thickness limbal explants.

More Related Videos

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
11:13

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

A Protocol for Harvesting Single-cell Suspension from Mouse Corneas
04:50

A Protocol for Harvesting Single-cell Suspension from Mouse Corneas

Published on: April 3, 2026

Related Experiment Videos

Last Updated: Jun 4, 2026

Isolation and Identification of Limbal Niche Cells
10:11

Isolation and Identification of Limbal Niche Cells

Published on: October 27, 2023

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
11:13

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Published on: April 7, 2016

A Protocol for Harvesting Single-cell Suspension from Mouse Corneas
04:50

A Protocol for Harvesting Single-cell Suspension from Mouse Corneas

Published on: April 3, 2026

  • Explants were cultured in Green medium without cholera toxin or feeder cells.
  • Cell growth, phenotype, and marker expression (immunocytochemistry, flow cytometry, RT-PCR) were analyzed.

    • Main Results:

    • Superficial limbal explants showed significantly higher successful growth rates (86%) compared to full-thickness explants (41%).
    • Superficial explant cultures exhibited smaller cell size and higher expression of epithelial and progenitor markers (CK3, MNF116, delta N p63α, ABCG2).
    • Surgical preparation of explants dramatically improved culture success, epithelial growth, and progenitor preservation while reducing fibroblast contamination.

    Conclusions:

    • Superficial limbal explants are superior to full-thickness explants for culturing human limbal epithelial cells.
    • Feeder-free, cholera toxin-free culture conditions support limbal epithelial cell growth and progenitor maintenance.
    • Surgical preparation techniques are critical for optimizing limbal explant culture for regenerative medicine applications.