Related Experiment Video
Updated: Jun 4, 2026
07:43
An Enzyme- and Serum-free Neural Stem Cell Culture Model for EMT Investigation Suited for Drug Discovery
Published on: August 23, 2016
The p38/MK2/Hsp25 pathway is required for BMP-2-induced cell migration
Cristina Gamell1, Antonio G Susperregui, Ora Bernard
1Departament de Ciències Fisiològiques II, Universitat de Barcelona, IDIBELL, L'Hospitalet de Llobregat, Spain.
Plos One
|February 8, 2011
Summary
Bone morphogenetic proteins (BMPs) regulate cell migration via Cdc42/PAK/LIMK1 and p38/MK2/Hsp25 pathways. These parallel signaling cascades are crucial for BMP-induced actin reorganization and cell movement.
Area of Science:
- Cell Biology
- Molecular Biology
- Developmental Biology
Background:
- Bone morphogenetic proteins (BMPs) are key regulators of development and cell functions, including cell migration.
- BMP-mediated cell migration involves Cdc42, LIMK1, and PAK activation.
- The precise signaling mechanisms underlying BMP-induced cell migration require further elucidation.
Purpose of the Study:
- To investigate the role of p38 MAPK signaling in BMP-2-induced cell migration and actin cytoskeleton remodeling.
- To determine the relationship between p38/MK2/Hsp25 and Cdc42/PAK/LIMK1 pathways in BMP signaling.
Main Methods:
- BMP-2 treatment of mesenchymal cells.
- Chemical inhibition and genetic ablation of p38 and MK2.
- Assessment of downstream effector activation and cell migration.
- Analysis of LIMK1 activation and Hsp25 phosphorylation and localization.
Main Results:
- BMP-2 induces cell migration and actin remodeling dependent on p38 activity.
- The p38/MK2/Hsp25 pathway is activated downstream of BMP receptors, independent of Cdc42/PAK.
- p38/MK2 activities do not significantly affect BMP-2-induced LIMK1 activation.
- Phosphorylated Hsp25 localizes with BMP receptors and its mutant form inhibits BMP-2-induced migration.
Conclusions:
- Cdc42/PAK/LIMK1 and p38/MK2/Hsp25 pathways act in parallel to regulate BMP-induced cell migration.
- These pathways modulate specific actin regulatory proteins, integrating responses for actin reorganization.
- The findings highlight the complex signaling network governing BMP-mediated cell motility.
More Related Videos
10:32Simple Establishment of a Vascularized Osteogenic Bone Marrow Niche Using Pre-Cast Poly(ethylene Glycol) (PEG) Hydrogels in an Imaging Microplate
Published on: May 19, 2023
10:43Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration
Published on: May 19, 2016
Related Concept Videos
01:07MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
01:19Interactions Between Signaling Pathways
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
01:09Cell Migration
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
01:19Cell Migration
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...