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Intra-laboratory validation of the Ridascreen® SET Total kit for detecting staphylococcal enterotoxins SEA to SEE in
A Ostyn1, F Guillier, A-L Prufer
1French Agency for Food, Environmental and Occupational Health & Safety (ANSES), Maisons-Alfort Laboratory for Food Safety, Unit Characterization of Toxins, National and European Union Reference Laboratory for Coagulase Positive Staphylococci Including Staphylococcus aureus, Maisons-Alfort Cedex, France.
Aim:
To determine the performance of the Ridascreen® SET Total kit, after sample extraction and concentration by dialysis, with regard to its use in official controls for staphylococcal enterotoxins under European Regulation (EC) No. 2073/2005 modified. This study was conducted on naturally contaminated cheese samples and compared with the results of the previously validated Vidas® SET2 kit.
Methods And Results:
The effectiveness of the Ridascreen® SET Total kit on naturally contaminated cheeses was compared to that of the Vidas® SET2 kit by applying the EN ISO 16140 standard. Sensitivity and specificity were also compared using spiked buffer solutions and cheese samples with SEA to SEE toxins.
Conclusions:
This study showed that the Ridascreen® SET Total kit is as effective as the Vidas® SET2 kit.
Significance And Impact Of The Study:
The Ridascreen® SET Total kit was found to specifically detect SEA to SEE in cheeses. The Ridascreen® SET Total can therefore be used to check the staphylococcal enterotoxin content and ensure consumer protection.
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