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Updated: Jun 4, 2026
Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
Comparative evaluation of bone regeneration using spherical and irregularly shaped granules of interconnected porous
Takayasu Kubo1, Kazuya Doi, Kazuhiko Hayashi
1Department of Advanced Prosthodontics, Division of Cervico-Gnathostomatology, Programs for Applied Biomedicine, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
Purpose:
Bone regeneration stimulated by two different shapes of interconnected porous calcium hydroxyapatite (IP-CHA) granules was evaluated in the mandibles of 3 beagle dogs.
Methods:
Deferent shapes of IP-CHA were used, spherical (spherical shaped IP-CHA granules) and irregular shapes (irregularly shaped IP-CHA granules). Two bone sockets (3mm in diameter and 5mm in depth) were prepared in the right edentulous mandible of each animal where right premolars had been extracted and sites healed for 3 months. The two types of IP-CHA were filled into the sockets to stimulate bone regeneration. New bone formation was evaluated histologically at 4, 8 and 12 weeks after filling.
Results:
At 4 weeks, little bone formation was apparent in any of the bony sockets. At 8 weeks, newly formed bone was detected between the granules but not in the pores. In contrast, at 12 weeks, bone formation was clearly observed not only between the granules but also inside the granule pores. Comparing the two sites at 8 and 12 weeks, more bone formation was detected in sites receiving irregularly shaped IP-CHA than in sites receiving spherical IP-CHA.
Conclusion:
These results indicate that use of irregularly shaped IP-CHA may enhance bone regeneration. The results of this preliminary study suggest that irregularly shaped IP-CHA granules may have more possible usefulness than spherically shaped granules as a scaffold for bone regeneration.
