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Updated: Jun 4, 2026
Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Published on: August 6, 2011
Rapid multiplex PCR assay for the simultaneous detection of the Brucella Genus, B. abortus, B. melitensis, and B.
Sanjay Kumar1, Urmil Tuteja, Kumari Sarika
1Division of Biotechnology, Defence Research and Development Establishment, Jhansi Road, Gwalior, Madhya Pradesh, India. drksanjay78@gmail.com
Abstract:
The routine identification and differentiation of Brucella species is a time-consuming and labor-intensive process, which frequently places personnel at risk of laboratoryacquired infection. Here, we describe the development of a rapid multiplex PCR assay for the confirmation of presumptive Brucella isolates. The assay was able to identify and differentiate major human pathogens, namely B. abortus, B. melitensis, and B. suis, in a single test of less than an hour and a half.
