[An experimental study on the toxicity of composite resins through tissue culture]

The study was to evaluate the toxicity of self curing resins in vitro. Fibroblasts (established cell L-929) were cultured in alpha-MEM with 4 different resins. Each resin was mixed by the instruction and filled in open end cylinder. These cylinder was placed at the centre of the dish. Millipore filter (pore size, 0.22Um) was also placed between the bottom of the cylinder and the dish. As a control group cylinder without resin was employed in this experiment. The culture dishes were then stored in 5% CO2 containing incubator for 5 days at the temperature of 36.6 degrees C. The results were observed microscopically. 1. Two groups showed least cell population compare to control group. (This indicated greater cytotoxicity). 2. Remaining two groups revealed lesser cell population compared to control group. (This indicated lesser cytotoxicity).