Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Steroid-binding peptides from dietary proteins.

M Sugano1, S Goto

  • 1Laboratory of Nutrition Chemistry, Kyushu University School of Agriculture, Fukuoka, Japan.

Journal of Nutritional Science and Vitaminology
|October 1, 1990
PubMed
Summary

The high-molecular weight fraction (HMF) of soybean protein effectively lowers cholesterol in rats. This HMF binds bile salts and increases steroid excretion, unlike the original soybean protein.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cepharanthine induces the proliferation of human dermal papilla cells and stimulates vascular endothelial growth factor expression through increased intracellular calcium mobilization and hypoxia-inducible factor activation.

Clinical and experimental dermatology·2020
Same author

Interactions of Dietary Fats and Proteins on Fatty Acid Composition of Immune Cells and LTB4 Production by Peritoneal Exudate Cells of Rats.

Bioscience, biotechnology, and biochemistry·2016
Same author

Impact of Dietary Protein on Polyunsaturated Fatty Acid Desaturation in Rats Fed Diets Rich in α-Linolenic Acid.

Bioscience, biotechnology, and biochemistry·2016
Same author

Comparative Effects of Dietary Palm Oil, Perilla Oil, and Soybean Oil on Lipid Profiles in Differently Aged Rats Fed on Hypercholesterolemic Diets.

Bioscience, biotechnology, and biochemistry·2016
Same author

Effects of Different Triglyceride Saturated Fatty Acids on Tissue Lipid Level, Fatty Acid Composition, Fecal Steroid Excretion, Prostacyclin Production, and Platelet Aggregation in Rats.

Bioscience, biotechnology, and biochemistry·2016
Same author

Comparisons of the Effects of Dietary Fats on Serum and Liver Lipid Levels of Rats.

Bioscience, biotechnology, and biochemistry·2016

Area of Science:

  • Nutritional Science
  • Biochemistry
  • Gastroenterology

Background:

  • Dietary proteins can influence cholesterol metabolism.
  • Soybean protein is a common dietary component with potential health benefits.
  • The specific fractions of digested proteins and their bioactivity require further investigation.

Purpose of the Study:

  • To investigate the hypocholesterolemic activity of the undigested high-molecular weight fraction (HMF) of soybean protein.
  • To compare the cholesterol-lowering effects of HMF with the parent soybean protein.
  • To explore the mechanisms underlying HMF's hypocholesterolemic activity, including bile salt binding and steroid excretion.

Main Methods:

  • Preparation of HMF from soybean protein through exhaustive digestion with microbial proteases or pepsin.
  • Administration of HMF and parent soybean protein to rats fed cholesterol-enriched diets.
  • In vitro assessment of HMF's binding capacity with bile salts.
  • Measurement of fecal excretion of neutral and acidic steroids.
  • Methanol extraction and further enzymatic degradation of HMF to assess activity determinants.
  • Comparison of bile acid binding capacity of HMF from soybean protein with other vegetable proteins.

Main Results:

  • The HMF of soybean protein demonstrated significant hypocholesterolemic activity in rats compared to the parent protein.
  • HMF exhibited in vitro binding with bile salts and markedly stimulated fecal excretion of neutral and acidic steroids.
  • Methanol extraction slightly reduced HMF activity, but the soluble fraction was not the primary active component; further protease degradation abolished activity.
  • HMF derived from soybean protein showed superior bile acid binding capacity compared to HMF from other vegetable proteins.

Conclusions:

  • The undigested high-molecular weight fraction of soybean protein possesses potent hypocholesterolemic properties.
  • HMF's cholesterol-lowering effect is mediated by enhanced bile salt binding and increased fecal steroid excretion.
  • Soybean protein HMF is a promising functional food ingredient for managing cholesterol levels.

Related Experiment Videos