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Updated: Jun 4, 2026
Isolation and Activation of Murine Lymphocytes
Published on: October 30, 2016
Rapid murine mixed lymphocyte cultures assessed by emergence of T-cell insulin receptors
T B Strom1, J H Helderman, R M Williams
1Department of Medicine, Harvard Medical School, Peter Bent Brigham Hospital, USA.
Murine T lymphocytes incubated in a mixed lymphocyte culture with allogeneic lymphocytes incompatible for theH-2 region or subregions thereof rapidly develop insulin receptors. In contrast, T cells cultured with syngeneic orH-2- andMlslocus compatible cells do not develop insulin-binding sites. The emergence of insulin receptors is probably an early premitotic event.
Murine T lymphocytes incubated in a mixed lymphocyte culture with allogeneic lymphocytes incompatible for theH-2 region or subregions thereof rapidly develop insulin receptors. In contrast, T cells cultured with syngeneic orH-2- andMlslocus compatible cells do not develop insulin-binding sites. The emergence of insulin receptors is probably an early premitotic event.
