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Updated: Jun 4, 2026

Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
Published on: November 20, 2018
Intra-H-2 recombination inH-2(b)/H-2 (t1) heterozygotes in the mouse : II. Characterization of recombinant
K W Beisel1, P K Haldar, H C Passmore
1Department of Zoology and Bureau of Biological Research, Rutgers University, 08903, New Brunswick, New Jersey.
Abstract:
Four cases of intra-H-2 recombination were detected during serological screening of 1066 backcross animals produced fromH-2(b)/H-2(t1) heterozygous mice. Three of the intra-H-2 recombinants received theK region fromH-2(t1) and theD region from theH-2(b) parental chromosome. The remaining recombinant received theK region from theH-2(b) parental chromosome and theD region fromH-2(t1). Three of the four recombinants have been developed into inbred lines TBR2, TBR3, and TBR4 and were assigned the haplotype designations at(2), at(3), and at(4). Ss typing revealed that TBR2 and TBR3 originated fromK- S interval crossover events, while the remaining two recombinants resulted from crossing over in theS- D interval.
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