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[Separation, culture and purification of rat brain microendothelial cell]
Lin-yu Tian1, Sheng-fu Li, Dong Zhou
1Department of Neurology, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To develop a separation method for brain microendothelial cells with the comparison of other ones.
Methods:
Twice enzymatic digestion and twice gradient centrifugation were applied to separate rat brain microendothelial cells. Then, immunomagnetic beads and Thy1.1 antibody were used respectively to purify the cultured cells.
Results:
Twice enzymatic digestion and twice gradient centrifugation could separate the cell successfully. High purification but low cell yield was obtained with immunomagnetic beads. The cells handled with Thy1.1 antibody had both higher purify coefficient and higher yield.
Conclusion:
The developed method could separate the brain microendothelial cells successfully.
