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[Effects of shexiang baoxin pill on angiogenesis in atherosclerosis plaque and ischemic myocardium]
Wei Shen1, Wei-hu Fan, Hai-ming Shi
1Department of Cardiology, Huashan Hospital, Fundan University, Shanghai 200040.
Objective:
To investigate the effects of Shexiang Baoxin Pill (SBP) in intervening atherosclerosis and myocardial infarction (AS-MI) in experimental animals, and inspect its influences on angiogenesis.
Methods:
Twenty male New-Zealand rabbits were made into AS-MI model, and randomly divided into 2 groups equally. Group A was fed with high-fat diet for control; Group B was fed with high-fat diet but intervened with SBP. The cardiac function and the positive area of plaque were determined. The CD34 positive response intensity at infarcted marginal zone and aorta vessel wall, and the capillary density of myocardium were measured by immunohistochemical staining. In addition, the protein expressions of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor-2 (VEGFR-2) were detected by Western blot.
Results:
Compared to Group A, the cardiac function was obviously improved (P<0.05) and the plaque positive area (%) was significantly decreased in Group B (45.82 +/- 3.68 vs 82.56 +/- 4.97, P<0.01). The CD34 positive response intensity and the capillary density as well as VEGF and VEGFR-2 expressions in infarcted marginal zone in Group A were higher than those in Group B (P<0.01); but these parameters at aorta vessel walls were lower in Group A than in Group B (P<0.01).
Conclusion:
SBP could advance the angiogenesis in the marginal zone of infarction, improve heart function, and embarrass angiogenesis in atherosclerotic plaque.
Insights
Shexiang Baoxin Pill (SBP) improved cardiac function and reduced atherosclerotic plaque in a rabbit model. SBP also promoted angiogenesis in infarcted heart tissue while inhibiting it in atherosclerotic plaques.
Area of Science:
- Cardiovascular Research
- Pharmacology
- Angiogenesis Studies
Context:
- Atherosclerosis and myocardial infarction (AS-MI) pose significant global health challenges.
- Investigating novel therapeutic interventions for AS-MI is crucial.
- Understanding the role of angiogenesis in AS-MI pathogenesis is an active area of research.
Purpose:
- To evaluate the therapeutic effects of Shexiang Baoxin Pill (SBP) on AS-MI in a rabbit model.
- To assess SBP's impact on cardiac function and atherosclerotic plaque formation.
- To investigate SBP's influence on angiogenesis in both infarcted myocardium and atherosclerotic lesions.
Summary:
- Shexiang Baoxin Pill (SBP) administration in rabbits with AS-MI led to significant improvements in cardiac function and a reduction in atherosclerotic plaque area compared to controls.
- Immunohistochemical analysis revealed that SBP enhanced CD34 expression and capillary density in the infarcted marginal zone.
- Western blot analysis indicated altered expressions of vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor-2 (VEGFR-2) in response to SBP treatment.
Impact:
- SBP demonstrates potential as a therapeutic agent for atherosclerosis and myocardial infarction.
- The findings suggest SBP modulates angiogenesis, promoting healing in damaged heart tissue while potentially hindering plaque progression.
- This study provides a basis for further research into SBP's mechanisms of action and clinical applicability.
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