Standard oral rehydration solution as a new storage medium for avulsed teeth

Area of Science:

• Dentistry
• Biomaterials Science
• Cell Biology

Background:

• Tooth avulsion necessitates prompt replantation.
• Maintaining periodontal ligament (PDL) cell viability is crucial for successful tooth reimplantation when immediate replantation is not feasible.
• Exploring alternative storage media is essential for improving outcomes in traumatic dental injuries.

Purpose of the Study:

• To evaluate the efficacy of standard Oral Rehydration Solution (ORS) as a novel storage medium for preserving PDL cell viability.
• To compare the effectiveness of ORS with established media like Hank's Balanced Salt Solution (HBSS) and tap water.

Main Methods:

• 75 premolar teeth were subjected to storage in standard ORS, HBSS, and tap water.
• Periodontal ligament (PDL) cells were isolated after storage.
• Cell viability was assessed using Trypan blue staining and analyzed via one-way ANOVA.

Main Results:

• Standard ORS demonstrated comparable PDL cell viability preservation to HBSS.
• Both standard ORS and HBSS showed significantly better results in maintaining PDL cell viability compared to tap water.
• No significant difference was found between HBSS and standard ORS in supporting PDL cell viability.

Conclusions:

• Standard ORS is a suitable and effective medium for preserving PDL cell viability.
• Due to its cost-effectiveness and widespread availability, standard ORS presents a practical alternative to HBSS for temporary tooth storage.
• The findings support the use of standard ORS in managing avulsed teeth when immediate replantation is not possible.