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Updated: Jun 4, 2026

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

3-D Cell Culture System for Studying Invasion and Evaluating Therapeutics in Bladder Cancer

Published on: September 13, 2018

Invadopodia formation by bladder tumor cells.

Mihoko Sutoh¹, Yasuhiro Hashimoto, Takahiro Yoneyama

¹Department of Biochemistry, Oyokyo Kidney Research Institute, Hirosaki, Aomori, Japan.

Oncology Research

|February 10, 2011

Summary

Human bladder tumor cells form invadopodia, actin-rich structures crucial for invasion and metastasis. This finding, observed in both cell lines and patient samples, reveals a key mechanism in bladder cancer progression.

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Published on: September 13, 2018

An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment

An Orthotopic Bladder Tumor Model and the Evaluation of Intravesical saRNA Treatment

Published on: July 28, 2012

Area of Science:

Oncology
Cell Biology
Cancer Metastasis

Background:

Bladder tumor recurrence and metastasis are major causes of mortality.
Tumor cell invasion, a critical step in metastasis, is mediated by invadopodia.
Invadopodia formation has been documented in various cancers but not previously in bladder tumors.

Purpose of the Study:

To investigate whether human bladder tumor cells form invadopodia.
To determine if invadopodia formation correlates with the invasive potential of bladder tumor cells.
To characterize the functional activity of invadopodia in bladder cancer.

Main Methods:

Confocal laser scanning microscopy was used to visualize invadopodia.
In vitro assays assessed Matrigel degradation activity of bladder tumor cell lines.
Primary tumor cells from patients with invasive bladder tumors were analyzed.

Main Results:

Invasive bladder tumor cell lines (YTS-1, T24) formed detectable invadopodia with high Matrigel degradation activity.
Noninvasive bladder tumor cell lines (RT4, KK-47) did not form detectable invadopodia.
Invadopodia from YTS-1 cells secreted matrix metalloproteases and degraded extracellular matrix, facilitating invasion. Primary tumor cells from invasive bladder tumors also formed invadopodia.

Conclusions:

Human bladder tumor cells are capable of forming functionally active invadopodia.
Invadopodia formation is associated with the invasive phenotype of bladder tumors.
Invadopodia represent a critical mechanism for bladder tumor cell invasion and potential metastasis.