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Inorganic Nitrogen Assimilation

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Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme nitrate reductase...

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Related Experiment Video

Updated: May 18, 2026

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

Published on: December 25, 2016

Technical updates to the bacterial method for nitrate isotopic analyses.

Matthew R McIlvin¹, Karen L Casciotti

¹Department of Marine Chemistry and Geochemistry, Woods Hole Oceanographic Institution, Woods Hole, Massachusetts 02543, United States.

Analytical Chemistry

|February 10, 2011

Summary

This study refines the denitrifier method for analyzing nitrate (NO(3)(-)) isotopes using bacterial conversion to nitrous oxide (N(2)O). Recent modifications improve precision and throughput for isotopic analysis.

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Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on ¹⁴NH₄⁺/¹⁵NH₄⁺ Analyses via Sequential Conversion to N₂O

Published on: October 7, 2020

Area of Science:

Environmental Science
Analytical Chemistry
Microbiology

Background:

The bacterial conversion of nitrate (NO(3)(-)) to nitrous oxide (N(2)O) is a key method for isotopic analysis.
Pseudomonas aureofaciens facilitates this conversion, preserving nitrogen (N) and oxygen (O) isotopic signatures.
Automation has previously enhanced the throughput of N(2)O isotopic analysis.

Purpose of the Study:

To present recent procedural modifications to the denitrifier method.
To demonstrate the effectiveness of these modifications for nitrate (NO(3)(-)) isotopic analysis.
To further increase the precision and throughput of nitrate (NO(3)(-)) isotopic analysis.

Main Methods:

Utilized the bacterial strain Pseudomonas aureofaciens (ATTC no. 13985).
Applied procedural modifications to the established denitrifier method.
Performed isotopic analysis on nitrous oxide (N(2)O) produced from nitrate (NO(3)(-)) conversion.

Main Results:

Demonstrated the effectiveness of several recent procedural modifications.
Showcased increased precision in nitrate (NO(3)(-)) isotopic analysis.
Achieved higher throughput for analyzing nitrate (NO(3)(-)) isotopes.

Conclusions:

Recent modifications enhance the denitrifier method for precise and high-throughput nitrate (NO(3)(-)) isotopic analysis.
The improved method offers significant advantages for environmental and geochemical studies.
Continued development advances the capabilities of bacterial-based isotopic analysis.