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Updated: Jun 4, 2026

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Ag@poly(m-phenylenediamine) core-shell nanoparticles for highly selective, multiplex nucleic acid detection.

Yingwei Zhang¹, Lei Wang, Jingqi Tian

¹State Key Lab of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.

Langmuir : the ACS Journal of Surfaces and Colloids

|February 10, 2011

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Researchers developed silver core@poly(m-phenylenediamine) shell nanoparticles (APCSNPs) for sensitive nucleic acid detection. This fluorescent sensing platform accurately identifies DNA sequences, even with single-base mismatches, and enables multiplex detection.

Area of Science:

Nanotechnology
Biochemistry
Analytical Chemistry

Background:

Core-shell nanoparticles offer unique properties for sensing applications.
Fluorescence quenching and recovery are key mechanisms in biosensing.
Nucleic acid detection requires high sensitivity and specificity.

Purpose of the Study:

To synthesize Ag@poly(m-phenylenediamine) core-shell nanoparticles (APCSNPs).
To establish APCSNPs as a novel fluorescent sensing platform for nucleic acid detection.
To evaluate the selectivity, reproducibility, and multiplexing capability of the APCSNP sensing system.

Main Methods:

One-step synthesis of APCSNPs via direct mixing of silver nitrate and m-phenylenediamine solutions.
Utilizing fluorescence quenching of dye-labeled ssDNA by APCSNPs.

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Last Updated: Jun 4, 2026

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

Published on: September 27, 2016

Monitoring fluorescence recovery upon hybridization with target DNA to detect dsDNA.

Main Results:

Successful synthesis of APCSNPs at room temperature.
Demonstrated high selectivity for DNA detection, down to single-base mismatch.
Exhibited good reproducibility in sensing results.
Showcased application in multiplex nucleic acid sequence detection.

Conclusions:

APCSNPs are effectively synthesized using a simple, one-step method.
The developed APCSNP platform provides a sensitive and selective approach for fluorescent nucleic acid detection.
This method holds promise for advanced diagnostics and genetic analysis, including multiplex detection.