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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
07:14

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

Published on: May 17, 2021

Is normal hematopoiesis maintained solely by long-term multipotent stem cells?

Marina Cavazzana-Calvo1, Alain Fischer, Frederic D Bushman

  • 1Department of Biotherapy, Hopital Necker Enfants-Malades, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France.

Blood
|February 10, 2011
PubMed

The understanding of the hierarchical organization of the human hematopoietic system is of major biologic and clinical significance. The validity of the conventional model in which hematopoiesis is solely maintained by a pool of multipotent long-term hematopoietic stem cells (LT-HSCs) has been recently challenged by several mouse studies. These new data point to the existence of a heterogeneous stem cell population that consists of distinct subsets of LT-HSCs, which include stem cells biased toward lineage-specific differentiation programs. This review attempts to discuss the balanced versus biased patterns of lineage output of human LT-HSCs gathered in 3 different gene therapy trials on the basis of vector integration site analysis by deep sequencing. The distribution of integration sites observed tends to support the validity of the revised model.

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