Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ESPeR-seq: Extremely Sensitive and Pure, End-to-end, RNA-seq library preparation.

bioRxiv : the preprint server for biology·2026
Same author

Prolonged Activity Deprivation Causes Pre- and Postsynaptic Compensatory Plasticity at Neocortical Excitatory Synapses.

eNeuro·2024
Same author

Progressive Circuit Hyperexcitability in Mouse Neocortical Slice Cultures with Increasing Duration of Activity Silencing.

eNeuro·2024
Same author

Altered 5-HT2A/C receptor binding in the medulla oblongata in the sudden infant death syndrome (SIDS): Part II. Age-associated alterations in serotonin receptor binding profiles within medullary nuclei supporting cardiorespiratory homeostasis.

Journal of neuropathology and experimental neurology·2024
Same author

Multiomic Analysis of Neuroinflammation and Occult Infection in Sudden Infant Death Syndrome.

JAMA neurology·2024
Same author

A transcriptional constraint mechanism limits the homeostatic response to activity deprivation in mammalian neocortex.

eLife·2023
Same journal

Analysis of strength degradation of coal and rock masses and stability of mined areas under long term immersion environment.

PloS one·2026
Same journal

Biogenic Silver-Selenium nanocomposite with anticancer activity and potent efficacy against vancomycin-resistant Staphylococcus aureus.

PloS one·2026
Same journal

Preparation and physicochemical characterization of a biodegradable chitosan/carboxymethyl cellulose hydrogel synthesized in NaOH/urea medium.

PloS one·2026
Same journal

Action-guilt, survivor-guilt, and depression in combat-related PTSD.

PloS one·2026
Same journal

Explainable machine learning for predicting activities of daily living at discharge in stroke patients: A retrospective study using SHAP interpretability.

PloS one·2026
Same journal

Deep learning based two-way feature depiction model for brain tumor detection.

PloS one·2026
See all related articles

Related Experiment Video

Updated: Jun 4, 2026

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
08:06

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Published on: February 15, 2021

A quantitative comparison of cell-type-specific microarray gene expression profiling methods in the mouse brain.

Benjamin W Okaty1, Ken Sugino, Sacha B Nelson

  • 1Department of Biology, Brandeis University, Waltham, Massachusetts, United States of America.

Plos One
|February 10, 2011
PubMed
Summary
This summary is machine-generated.

Comparing five methods for isolating neural cells for gene expression analysis, this study found Laser Capture Microdissection (LCM) and Translating Ribosome Affinity Purification (TRAP) had higher contamination. Immunopanning (PAN) showed stress gene activation, unlike FACS and Manual sorting.

More Related Videos

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling
10:06

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Published on: November 13, 2011

A Comparative Approach for Quantitative Cell Counting Studies in Widely Different Mammalian Brains
07:14

A Comparative Approach for Quantitative Cell Counting Studies in Widely Different Mammalian Brains

Published on: January 16, 2026

Related Experiment Videos

Last Updated: Jun 4, 2026

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions
08:06

Microdissection of Mouse Brain into Functionally and Anatomically Different Regions

Published on: February 15, 2021

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling
10:06

Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling

Published on: November 13, 2011

A Comparative Approach for Quantitative Cell Counting Studies in Widely Different Mammalian Brains
07:14

A Comparative Approach for Quantitative Cell Counting Studies in Widely Different Mammalian Brains

Published on: January 16, 2026

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genomics

Background:

  • Analyzing gene expression in specific neural populations is crucial for understanding brain function and cellular phenotypes.
  • The heterogeneity of brain cell types presents significant challenges for isolating pure populations for molecular analysis.
  • Existing cell purification methods may introduce biases, such as mRNA contamination or stress-induced gene expression changes.

Purpose of the Study:

  • To compare the repeatability, contamination levels, and stress-gene induction of five distinct neural cell purification methods.
  • To identify the most reliable method for obtaining pure neural cell populations for accurate gene expression profiling.
  • To assess the impact of different purification techniques on cellular gene expression profiles.

Main Methods:

  • Five methods were evaluated: Laser Capture Microdissection (LCM), Translating Ribosome Affinity Purification (TRAP), Immunopanning (PAN), Fluorescence Activated Cell Sorting (FACS), and manual sorting.
  • Methods were compared based on repeatability, contamination from non-target cells, and expression of stress-responsive genes.
  • Gene expression analysis was performed using microarrays.

Main Results:

  • All five methods demonstrated high repeatability.
  • Laser Capture Microdissection (LCM) and Translating Ribosome Affinity Purification (TRAP) exhibited significantly higher levels of cell-type contamination compared to other methods.
  • Immunopanning (PAN) samples showed increased expression of apoptosis and stress-related genes, while Fluorescence Activated Cell Sorting (FACS) and Manual sorting did not.

Conclusions:

  • No single method is ideal for all neural expression profiling applications; method choice depends on experimental goals.
  • Fluorescence Activated Cell Sorting (FACS) and Manual sorting appear preferable when minimizing stress-related gene induction is critical.
  • Differences in results, particularly with Translating Ribosome Affinity Purification (TRAP), may stem from targeting actively translated mRNAs versus all transcribed mRNAs.