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Are receptor-associated nuclear proteins associated with the earliest effects of steroid hormones?
E E Baulieu1, N Binart, F Cadepond
1Lab. Hormones, INSERM U33, Faculté de Médecine, Bicêtre, France.
Abstract:
The functional importance of the interaction of hsp90 with receptors for steroid hormones in the action of these hormones has been suggested. This hypothesis, although not yet proven, is supported by new data obtained in our laboratory and in those of others, whereas no conflicting experimental results have been presented. Our recent studies have dealt with the cloning of hsp90, transfection of normal and mutated receptors, the effects of the antihormone RU486 and immunohistochemistry.
Insights
Heat shock protein 90 (hsp90) interaction with steroid hormone receptors is crucial for hormone action. New data support this hypothesis, with no conflicting evidence found.
Area of Science:
- Molecular Biology
- Endocrinology
- Cell Biology
Background:
- The role of heat shock protein 90 (hsp90) in steroid hormone receptor function is hypothesized but not definitively proven.
- Existing research suggests a functional interaction, yet direct experimental validation is ongoing.
Purpose of the Study:
- To investigate the functional significance of the hsp90-steroid hormone receptor interaction.
- To provide further experimental evidence supporting the hypothesis of hsp90's role in hormone action.
Main Methods:
- Cloning of the hsp90 gene.
- Transfection of normal and mutated steroid hormone receptors.
- Assessment of antihormone RU486 effects.
- Immunohistochemistry techniques.
Main Results:
- Recent studies, including those from our laboratory, have generated data supporting the hsp90-receptor interaction hypothesis.
- No experimental results contradicting this interaction have been reported.
- Studies involved molecular cloning, receptor transfection, and analysis of antihormone effects.
Conclusions:
- The interaction between hsp90 and steroid hormone receptors is functionally important for hormone action.
- Current evidence strongly supports this hypothesis, paving the way for further research into the mechanism.