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Related Experiment Videos

Ethylene: receptors and action.

M A Hall1, C P Connern, N V Harpham

  • 1Department of Biological Sciences, University College of Wales, Aberystwyth, Dyfed, UK.

Symposia of the Society for Experimental Biology
|January 1, 1990
PubMed
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Researchers purified an ethylene binding protein from Phaseolus vulgaris, finding it in various plants. Ethylene treatment increased binding sites in abscission zones, suggesting a role in plant hormone responses.

Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Ethylene is a key plant hormone regulating growth and development.
  • Understanding ethylene perception is crucial for crop improvement.

Purpose of the Study:

  • To characterize and purify the ethylene binding protein from Phaseolus vulgaris.
  • To investigate the distribution and regulation of ethylene binding sites in plants.
  • To assess the potential of Arabidopsis mutants for ethylene receptor studies.

Main Methods:

  • Purification of ethylene binding protein from Phaseolus vulgaris cotyledons.
  • Development of polyclonal antibodies for protein detection.
  • Direct binding assays and immunological studies.
  • Analysis of ethylene binding sites in various plant species and mutants.

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Main Results:

  • An ethylene binding protein was purified from Phaseolus vulgaris.
  • Homologous proteins were detected in peas, tomatoes, and Arabidopsis.
  • Ethylene treatment increased binding site abundance in Phaseolus abscission zones.
  • Two classes of ethylene binding sites were identified in peas, Arabidopsis, and rice, differing in association/dissociation rates.
  • Arabidopsis mutants insensitive to ethylene were assessed for receptor deficiency.

Conclusions:

  • High association rate binding sites are likely functional ethylene receptors.
  • Low association rate binding sites may represent receptor precursors or internalized receptors.
  • Ethylene binding proteins play a conserved role in plant hormone signaling across species.