Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...

Karyotyping

Karyotyping

Describing the number and physical features of chromosomes can reveal abnormalities that underlie genetic diseases. This description is facilitated by special staining techniques that produce a particular banding pattern on each chromosome. State-of-the-art techniques make this approach even more powerful, enabling the detection of individual genes that cause disease.A Simple Chromosome Staining Technique Provides Valuable Scientific InsightSome genetic diseases can be detected by looking at...

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Zinc-polydopamine nanozyme promotes mitochondrial biogenesis and alleviates inflammation and muscle atrophy during the perioperative period of surgery.

Journal of materials science. Materials in medicine·2026

Same author

Molecular mechanism of <i>miR-27a-3p</i> targeting <i>FBXW7</i> regulating the malignant behavior of osteosarcoma cells.

Translational cancer research·2026

Same author

Rotational 3D printing of active-passive filaments and lattices with programmable shape morphing.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

"It's a Donut!" - Veterans' perspectives of lung cancer screening with low-dose computed tomography.

Patient education and counseling·2026

Same author

Interpretable Deep Learning for Single-Molecule Nanopore Fingerprinting Using Physics-Guided Preprocessing.

ACS sensors·2026

Same author

MicroRNA spatial profiling for assessing drug efficacy in <i>BRCA1</i> -related triple-negative breast tumors.

bioRxiv : the preprint server for biology·2026

Same journal

Pressure-Related Challenges and Strategic Approaches in Lithium Metal Sulfide all-Solid-State Batteries.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Dual Regulatory Functions of Classical Zinc Finger Clusters from Myeloid Zinc Finger-1.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same journal

A Selectfluor-based Polonovski Rearrangement Leading to Novel Entities for Synthetic and Medicinal Applications.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Relay Approach: A Convergent Synthesis of Key Fragments en route to (+)-Neosorangicin A.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Working Under Pressure: Empirical Findings on the Challenges Facing PhD Students in Chemistry.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

Same journal

Charge Resonance Interaction in Aromatic Trimer Radical Cations Revealed by IR Spectroscopy: The Case of Pyrrole Homo- and Heterotrimers.

Chemistry (Weinheim an der Bergstrasse, Germany)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 4, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Genotyping by alkaline dehybridization using graphically encoded particles.

Huaibin Zhang¹, Adam J DeConinck, Scott C Slimmer

¹Department of Chemistry, University of Illinois at Urbana-Champaign, 600 S. Mathews Ave., Urbana, IL 61801, USA.

Chemistry (Weinheim an Der Bergstrasse, Germany)

|February 10, 2011

Summary

This summary is machine-generated.

This study introduces a novel nonenzymatic DNA genotyping method using alkaline-induced dehybridization kinetics. This approach accurately distinguishes between perfectly matched and single-base mismatched DNA, enabling precise genetic analysis.

More Related Videos

Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

Related Experiment Videos

Last Updated: Jun 4, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Competitive Genomic Screens of Barcoded Yeast Libraries

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

Area of Science:

Biochemistry
Molecular Biology
Genetics

Background:

Accurate DNA genotyping is crucial for genetic analysis and diagnostics.
Distinguishing single-base mismatches in DNA duplexes presents a significant challenge in molecular biology.

Purpose of the Study:

To develop a nonenzymatic, isothermal method for DNA genotyping.
To utilize kinetic differences in DNA duplex dehybridization for mismatch discrimination.

Main Methods:

Fabrication of multifunctional encoded hydrogel particles using microfluidic stop-flow lithography.
Incorporation of allele-specific oligonucleotide (ASO) probes for simultaneous probing of two alleles.
Monitoring duplex dehybridization rates in alkaline solutions via fluorescence microscopy.

Main Results:

Demonstrated discrimination between perfectly matched (PM) and single-base mismatched (MM) DNA duplex dehybridization rates.
Successfully differentiated dehybridization kinetics for two distinct perfectly matched duplexes and two mismatched duplexes on a single particle.
Showcased temperature dependence of dehybridization rates and compensation using a gradient pH field.

Conclusions:

Alkaline-induced dehybridization kinetics offer a robust method for DNA genotyping and mismatch detection.
The developed hydrogel particle system enables simultaneous, high-certainty discrimination of multiple DNA targets.
This nonenzymatic approach provides a sensitive and adaptable platform for genetic analysis.