Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spectroscopic Studies of the Modulation of Metabolic Activity of HBE Cells by Doxorubicin Hydrochloride-Loaded SW480-Derived Exosomal Nanocarriers.

Cancers·2026
Same author

Mitochondrial Dynamics in Neurodegenerative Diseases: Unraveling the Role of Fusion and Fission Processes.

International journal of molecular sciences·2023
Same author

Current progress, strategy, and prospects of PD-1/PDL-1 immune checkpoint biosensing platforms for cancer diagnostics, therapy monitoring, and drug screening.

Biosensors & bioelectronics·2023
Same author

Effects of Supplementation with an Herbal Mixture on the Antioxidant Capacity of Milk.

Animals : an open access journal from MDPI·2023
Same author

DNA Aptamer Beacon Probe (ABP) for Monitoring of Adenosine Triphosphate Level in SW480 Cancer Cells Treated with Glycolysis Inhibitor 2-Deoxyglucose.

International journal of molecular sciences·2023
Same author

Effective Optical Image Assessment of Cellulose Paper Immunostrips for Blood Typing.

International journal of molecular sciences·2022

Related Experiment Video

Updated: Jun 4, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

Double-shell gold nanoparticle-based DNA-carriers with poly-L-lysine binding surface.

Magdalena Stobiecka1, Maria Hepel

  • 1Department of Chemistry, State University of New York at Potsdam, Potsdam, NY 13676, USA.

Biomaterials
|February 11, 2011
PubMed
Summary

Poly-l-lysine (PLL) coatings on gold nanoparticles (AuNP) show strong electrostatic interactions, enabling their use in non-toxic gene delivery systems. These functionalized nanoparticles effectively attract DNA, offering a safer alternative to thiol-based carriers.

More Related Videos

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery
10:38

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Published on: January 15, 2018

Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery
09:09

Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery

Published on: June 23, 2020

Related Experiment Videos

Last Updated: Jun 4, 2026

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures
08:30

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Published on: January 19, 2019

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery
10:38

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Published on: January 15, 2018

Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery
09:09

Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery

Published on: June 23, 2020

Area of Science:

  • Nanotechnology and Materials Science
  • Biomaterials Engineering
  • Molecular Interactions

Background:

  • Gold nanoparticles (AuNP) are explored for various biomedical applications, including gene delivery.
  • Poly-l-lysine (PLL) is a candidate for functionalizing AuNP due to its polycationic nature.
  • Direct PLL binding to AuNP is weak at neutral pH, necessitating alternative surface modification strategies.

Purpose of the Study:

  • To investigate the interactions between poly-l-lysine (PLL) and carboxylated gold nanoparticles (AuNP@Cit).
  • To characterize the resulting double-shell nanoparticles (AuNP@Cit/PLL) for potential gene delivery applications.
  • To assess the non-toxicity and efficacy of these functionalized nanoparticles as gene carriers.

Main Methods:

  • Synthesis of double-shell nanoparticles: AuNP@Cit/PLL via electrostatic interactions.
  • Characterization using UV-Vis spectroscopy (local surface plasmon band shifts) and Resonance Elastic Light Scattering (RELS).
  • Stoichiometry and binding affinity evaluation using RELS titration.
  • Molecular dynamics simulations to probe inter-particle interactions and hydrogen bonding.

Main Results:

  • Strong electrostatic interactions observed between PLL and AuNP@Cit in the pH range of 5-9, indicated by bathochromic shifts and increased RELS intensity.
  • Hyper-Langmuirian interaction model with a stoichiometric coefficient of 1.35 (PLL:AuNP@Cit).
  • Shift in PLL deprotonation constant (pKa = 11.6 for bound PLL vs. 10.48 for free PLL).
  • AuNP@Cit/PLL nanoparticles demonstrated DNA attraction, confirmed by RELS measurements, validating their potential for gene delivery.
  • Absence of toxic thiols in the synthesized nanoparticles.

Conclusions:

  • The AuNP@Cit/PLL system provides a robust, non-toxic platform for gene delivery applications.
  • Electrostatic interactions drive the strong binding of PLL to carboxylated AuNP.
  • The developed nanoparticles offer a promising alternative to thiol-functionalized carriers due to their inherent safety profile.