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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Updated: Jun 4, 2026

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

Transcriptomes of the B and T lineages compared by multiplatform microarray profiling.

Michio W Painter1, Scott Davis, Richard R Hardy

  • 1Department of Pathology, Harvard Medical School, Boston, MA 02215, USA.

Journal of Immunology (Baltimore, Md. : 1950)
|February 11, 2011
PubMed
Summary
This summary is machine-generated.

This study reveals widespread transcriptional differences between T and B lymphocytes, the two main branches of adaptive immunity. These distinctions emerge gradually during cell differentiation and are largely unique to each lineage.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genomics

Background:

  • T and B lymphocytes are key adaptive immune cells originating from a common precursor.
  • T cells mediate cell-mediated immunity, while B cells produce antibodies.
  • Understanding transcriptional differences is crucial for deciphering immune cell function.

Purpose of the Study:

  • To provide a genomewide perspective on transcriptional differences between T and B lymphocytes.
  • To identify robust differential gene expression signatures distinguishing these cell types.

Main Methods:

  • Utilized combinatorial profiling across multiple microarray platforms.
  • Leveraged the Immunological Genome Project gene expression database for comprehensive analysis.

Main Results:

  • Found pervasive and statistically significant differential gene expression between T and B cells.
  • Observed that distinguishing transcriptional characteristics develop gradually throughout differentiation.
  • Identified few signature genes uniquely expressed in T or B lineages; most are shared across immune cells.

Conclusions:

  • Transcriptional divergence between T and B cells is extensive and acquired progressively.
  • The majority of gene expression differences are lineage-specific, acquired during differentiation.
  • Few T- or B-cell specific genes exist; most signature genes are broadly expressed in immune cells.