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DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
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Related Experiment Video

Updated: Jun 4, 2026

Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
08:21

Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry

Published on: September 1, 2019

Cell-cycle-dependent active thermal bystander effect (ATBE).

Martin Purschke1, R Rox Anderson, David Zurakowski

  • 1Massachusetts General Hospital/Harvard Medical School, Wellman Center for Photomedicine, Boston, Massachusetts 02114, USA. mpurschke@partners.org

Lasers in Surgery and Medicine
|February 11, 2011
PubMed
Summary
This summary is machine-generated.

The active thermal bystander effect (ATBE) is dependent on cell division. Actively dividing cells, not quiescent cells, are required for ATBE, suggesting potential applications in targeting fast-growing cells like tumors.

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Area of Science:

  • Cell Biology
  • Thermal Biology
  • Cancer Research

Background:

  • The active thermal bystander effect (ATBE) is a phenomenon where heated cells can induce damage in nearby non-heated cells.
  • Understanding the factors influencing ATBE is crucial for its potential therapeutic applications.

Purpose of the Study:

  • To investigate whether the active thermal bystander effect (ATBE) is dependent on the cell cycle.
  • To compare ATBE in dividing versus non-dividing human cells.

Main Methods:

  • Direct heating of dividing cells for 10 minutes.
  • Co-culture of heated cells with various bystander cell types for 24 hours.
  • Assessment of bystander cell viability using MTT assay and cell-cycle analysis via flow cytometry.

Main Results:

  • Dividing fibroblasts and preadipocytes exhibited significant ATBE, with approximately 10% loss of cell viability at 40-48°C.
  • Non-dividing fibroblasts and mature adipocytes did not generate ATBE within the tested temperature range.
  • A statistically significant difference in ATBE was observed between dividing and non-dividing cell subpopulations for both cell types.

Conclusions:

  • The active thermal bystander effect (ATBE) is a cell-cycle-dependent process.
  • Actively dividing cells are necessary for the generation of ATBE.
  • The cell-cycle dependency of ATBE suggests potential for selectively targeting rapidly proliferating cells, such as cancer cells.