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Footrot on a sheep breeding farm in the Himalayan state of Jammu and Kashmir
1Division of Veterinary Microbiology & Immunology, Faculty of Veterinary Sciences & Animal Husbandry, S.K. University of Agricultural Sciences & Technology of Jammu, RS Pura-181 102, Jammu, Jammu and Kashmir, India. aniltaku@hotmail.com
Abstract:
In the present study ovine footrot was detected clinically on a sheep farm in the Himalayan state of Jammu and Kashmir. Dichelobacter nodosus was confirmed by culture and polymerase chain reaction (PCR) using species-specific 16S ribosomal RNA primers. When cultured, the organism appeared as flat colourless colonies having a fine granulated structure with irregular margins, and showing characteristic Gram-negative rods with swollen ends. Detection by PCR from cultured bacteria resulted in amplification of a 783 base pairs (bp) product. Serogrouping by multiplex PCR using group (A-I)-specific primers revealed the presence of serogroup B-specific bands of 283 bp.
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