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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometry: Overview01:19

Mass Spectrometry: Overview

Mass spectrometry is an analytical technique used to determine the molecular mass and molecular formula of a compound. The basic principle of mass spectrometry is to generate ions from the analyte molecule and measure these ion abundances against their molecular mass. One common type of ionization, known as electron ionization or EI, bombards the analyte molecules in the gas phase with high-energy electron beams. The electron beams displace an electron from the molecule and leave behind a...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Related Experiment Video

Updated: Jun 4, 2026

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
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Biological signalling activity measurements using mass spectrometry.

Pedro R Cutillas1, Claus Jørgensen

  • 1Analytical Signalling Laboratory, Centre for Cell Signalling, Institute of Cancer, Bart's and the London School of Medicine, Queen Mary University of London, Charterhouse Square, London EC1M 6BQ, UK. p.cutillas@qmul.ac.uk

The Biochemical Journal
|February 12, 2011
PubMed
Summary

Mass spectrometry techniques enable deep analysis of cell signaling networks. Multiplex quantification and phosphoproteomics offer complementary ways to understand biological signaling in health and disease.

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Area of Science:

  • Biochemistry and Molecular Biology
  • Systems Biology
  • Analytical Chemistry

Background:

  • Mass spectrometry (MS) techniques are advancing rapidly in performance and robustness.
  • These advancements allow for in-depth and accurate interrogation of biological signaling networks.

Purpose of the Study:

  • To review how MS-based multiplex quantification of kinase activities and phosphoproteomics can assess biological signaling.
  • To discuss the broader application of these analytical concepts for understanding system-level biology.

Main Methods:

  • Review of mass spectrometry-based multiplex quantification of kinase activities.
  • Review of phosphoproteomics techniques for assessing biological signaling.
  • Discussion of analytical concepts for quantifying kinase signaling.

Main Results:

  • MS-based multiplex quantification and phosphoproteomics provide complementary approaches to study signaling.
  • These methods offer unprecedented depth and accuracy in analyzing signaling networks.

Conclusions:

  • Wider application of these MS-based analytical concepts will enhance understanding of normal and disease biology.
  • A systems-level view of kinase signaling is achievable through advanced MS techniques.