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Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

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Related Experiment Video

Updated: Jun 4, 2026

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
09:52

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

Published on: January 31, 2019

Imaging of nucleic acids with atomic force microscopy.

Yuri L Lyubchenko1, Luda S Shlyakhtenko, Toshio Ando

  • 1Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198-6025, USA. ylyubchenko@unmc.edu

Methods (San Diego, Calif.)
|February 12, 2011
PubMed
Summary

Atomic force microscopy (AFM) enables reliable imaging of DNA and RNA nanostructures. Recent advances focus on surface modification and time-lapse AFM for dynamic nucleic acid studies.

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Last Updated: Jun 4, 2026

Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging
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Area of Science:

  • Nanotechnology
  • Biophysics
  • Molecular Biology

Background:

  • Atomic force microscopy (AFM) is crucial for nanotechnology, particularly in DNA and RNA nanotechnology.
  • Recent methodological advancements allow for reliable and reproducible imaging of diverse DNA and RNA nanostructures.
  • Mica surface modification techniques are key to achieving high-quality AFM imaging.

Purpose of the Study:

  • To review advances in AFM for imaging DNA and RNA nanostructures.
  • To provide background on established DNA AFM imaging for improving RNA imaging techniques.
  • To highlight the potential of AFM for studying nucleic acid dynamics.

Main Methods:

  • Surface modification of mica substrates for enhanced imaging.
  • Utilizing Atomic Force Microscopy (AFM) for high-resolution imaging of nucleic acids.
  • Employing time-lapse AFM to capture dynamic processes at the nanoscale.

Main Results:

  • Demonstrated reliable and reproducible imaging of various DNA and RNA structures and nanostructures.
  • Illustrated examples of imaging different DNA and RNA configurations.
  • Showcased the application of time-lapse AFM for observing nanoscale dynamics.

Conclusions:

  • AFM is a powerful tool for visualizing DNA and RNA nanostructures.
  • Surface preparation methods significantly impact imaging quality.
  • Time-lapse AFM offers new avenues for understanding nucleic acid dynamics.