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A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

Published on: October 4, 2019

Pathway engineering for functional isoprenoids.

Norihiko Misawa¹

¹Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Suematsu, Nonoichi-machi, Ishikawa 921-8836, Japan. n-misawa@ishikawa-pu.ac.jp

Current Opinion in Biotechnology

|February 12, 2011

Summary

Pathway engineering advances the production of valuable isoprenoids using microbial and plant hosts. Recent research focuses on engineering genes and enzymes for compounds like carotenoids in Escherichia coli and Saccharomyces cerevisiae.

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Area of Science:

Metabolic Engineering
Synthetic Biology
Biotechnology

Background:

Pathway engineering is crucial for producing target compounds in heterologous organisms.
Isoprenoids, including terpenes and carotenoids, are valuable compounds with diverse applications.
Recent advancements focus on microbial and plant hosts for efficient biosynthesis.

Purpose of the Study:

To review recent pathway engineering research for functional isoprenoid production.
To highlight the elucidation of biosynthesis genes and enzymes.
To cover advancements in microbial (Escherichia coli, Saccharomyces cerevisiae) and plant hosts.

Main Methods:

Engineering of biosynthetic pathways in microbial hosts.
Genetic manipulation and optimization of gene expression.
Utilizing plants as hosts for isoprenoid production.

Main Results:

Successful production of various isoprenoids like monoterpenes, sesquiterpenes, diterpenes, triterpenes, and carotenoids.
Identification and characterization of key biosynthesis genes and enzymes.
Demonstrated efficacy of Escherichia coli and Saccharomyces cerevisiae as microbial hosts.

Conclusions:

Pathway engineering is a powerful tool for sustainable isoprenoid production.
Microbial hosts offer advantages due to amenability to genetic manipulation.
Plant-based production systems are also promising for specific isoprenoids like carotenoids.