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Related Concept Videos

Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...
In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...

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Updated: Jun 4, 2026

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
11:06

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Published on: February 24, 2014

Genomic instability in induced stem cells.

C E Pasi1, A Dereli-Öz, S Negrini

  • 1Department of Experimental Oncology, IFOM-IEO Campus, Istituto Europeo di Oncologia, Milan, Italy.

Cell Death and Differentiation
|February 12, 2011
PubMed
Summary
This summary is machine-generated.

Stem cell reprogramming, often using c-Myc, can cause genomic instability and DNA damage. This instability is linked to c-Myc and may explain why p53 mutations aid reprogramming.

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Last Updated: Jun 4, 2026

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells
11:06

Identifying DNA Mutations in Purified Hematopoietic Stem/Progenitor Cells

Published on: February 24, 2014

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine
07:46

Generation and Maintenance of Primate Induced Pluripotent Stem Cells Derived from Urine

Published on: July 28, 2023

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
09:07

Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency

Published on: June 10, 2018

Area of Science:

  • Cell biology
  • Genetics
  • Oncology

Background:

  • Stem cell reprogramming offers potential for treating human diseases.
  • Proto-oncogenes like c-Myc are commonly used in reprogramming protocols.
  • Previous research linked oncogene expression to DNA replication stress and genomic instability.

Purpose of the Study:

  • To investigate whether stem cell reprogramming induces genomic instability.
  • To determine the role of c-Myc in reprogramming-induced genomic aberrations.

Main Methods:

  • Examined stem cells generated using three different reprogramming protocols.
  • Utilized comparative genomic hybridization (CGH) to detect genomic alterations.
  • Assessed the dependency of genomic aberrations on c-Myc expression.

Main Results:

  • Stem cells derived from reprogramming exhibited genomic deletions and amplifications.
  • The observed genomic aberrations were characteristic of oncogene-induced DNA replication stress.
  • Genomic instability was significantly dependent on c-Myc expression.

Conclusions:

  • Stem cell reprogramming can lead to significant genomic instability.
  • c-Myc expression is a key driver of these genomic aberrations.
  • The findings suggest a mechanism explaining why p53 inactivation facilitates stem cell reprogramming.