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Updated: Jun 4, 2026

A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl
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Mouse granzyme K has pro-inflammatory potential.

L T Joeckel1, R Wallich, P Martin

  • 1Metschnikoff Laboratory, Max-Planck-Institut für Immunbiologie, Freiburg, Germany.

Cell Death and Differentiation
|February 12, 2011
PubMed
Summary
This summary is machine-generated.

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Granzymes K (gzmK) from T-killer cells control lymphocytic choriomeningitis virus by inducing interleukin-1β in macrophages, not through cell killing.

Area of Science:

  • Immunology
  • Virology

Background:

  • Granzymes (gzms) are T-killer (Tc) cell components traditionally linked to apoptosis.
  • Emerging evidence indicates gzms have non-cytotoxic roles in host defense.

Purpose of the Study:

  • To investigate the role of granzyme K (gzmK) in T-killer cell-mediated control of lymphocytic choriomeningitis virus (LCMV).
  • To determine if gzmK exhibits pro-apoptotic activity or other functions during viral infection.

Main Methods:

  • Compared LCMV infection control in wild-type and granzyme A/B-deficient mice.
  • Assessed gzmK protein levels in T-killer cells.
  • Evaluated the effect of recombinant gzmK and T-cell-secreted gzmK on macrophage interleukin-1β production.
  • Investigated the role of the P2X(7) receptor and IL-1Ra (Anakinra) in gzmK-mediated responses.

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Last Updated: Jun 4, 2026

A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl
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Published on: November 28, 2014

A High Yield and Cost-efficient Expression System of Human Granzymes in Mammalian Cells
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A High Yield and Cost-efficient Expression System of Human Granzymes in Mammalian Cells

Published on: June 10, 2015

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
07:56

Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

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Main Results:

  • T-killer cells from both wild-type and gzm A/B-deficient mice expressed similar gzmK levels and controlled LCMV infection.
  • Recombinant or T-cell-derived gzmK lacked pro-apoptotic activity.
  • GzmK induced interleukin-1β secretion from primary mouse macrophages independently of P2X(7).
  • IL-1Ra treatment impaired LCMV elimination without affecting cytotoxic T-killer cell generation.

Conclusions:

  • T-killer cells control LCMV infection through non-cytotoxic mechanisms involving gzmK.
  • GzmK plays a crucial role in host defense by modulating macrophage inflammatory responses.
  • These findings reveal a novel non-cytotoxic function of granzymes in antiviral immunity.