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LSPR Imaging: Simultaneous Single Nanoparticle Spectroscopy and Diffusional Dynamics.

Julia M Bingham¹, Katherine A Willets, Nilam C Shah

¹Department of Chemistry, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208.

The Journal of Physical Chemistry. C, Nanomaterials and Interfaces

|September 28, 2011

Summary

This study introduces a new imaging method to track silver nanoparticles (Ag NPs) and measure their light scattering spectra simultaneously. This allows for real-time determination of nanoparticle diffusion coefficients, advancing fundamental and biological research.

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Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

Area of Science:

Nanotechnology
Spectroscopy
Biophysics

Background:

Localized surface plasmon resonance (LSPR) is sensitive to nanoparticle environment and size.
Characterizing nanoparticle dynamics and spectral properties simultaneously is challenging.
Existing methods often lack the parallel processing capability for multiple nanoparticles.

Purpose of the Study:

To develop and demonstrate a wide-field LSPR imaging method for parallel characterization of moving silver nanoparticles.
To enable real-time coupling of spectral and diffusion information for individual nanoparticles.
To determine single particle diffusion coefficients from combined spectral and trajectory data.

Main Methods:

Utilized a liquid crystal tunable filter (LCTF) for wide-field LSPR imaging.
Measured scattering spectra of multiple silver nanoparticles in parallel.
Simultaneously tracked the motion of individual nanoparticles to obtain trajectories.
Calculated single particle diffusion coefficients from the acquired trajectories.

Main Results:

Successfully measured LSPR scattering spectra of multiple moving Ag nanoparticles concurrently.
Obtained real-time trajectories and spectral data for individual Ag nanoprisms.
Determined single particle diffusion coefficients for the tracked Ag nanoprisms.
Demonstrated the feasibility of coupling diffusion and spectral information.

Conclusions:

The developed LSPR imaging method enables parallel characterization of moving nanoparticles.
Real-time coupling of spectral and diffusion data is a significant advancement for nanoparticle studies.
This technique has broad applications in fundamental science and biology, including cell membrane studies and nanoparticle growth mechanisms.