Related Experiment Video
Updated: Jun 4, 2026
07:52
Multiplexed Fluorescent Immunohistochemical Staining, Imaging, and Analysis in Histological Samples of Lymphoma
Published on: January 9, 2019
Immunohistochemistry in leukemias and lymphomas
M Jaramillo1, C Rangel, T Grogan
1Department of Pathology, Arizona Health Science Center, University of Arizona, Tucson, AZ.
Methods in Molecular Medicine
|February 12, 2011
Summary
Immunophenotyping of lymphoid and hematologic neoplasms enhances diagnosis by identifying specific cell markers. These unique profiles aid in classifying lymphomas and leukemias, improving patient outcomes.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Characterizing antigenic and immunologic properties of lymphoid and hematologic neoplasms advances disease biology understanding.
- Immunophenotyping identifies specific lineage aberrancies indicative of malignant transformation.
- Diagnostic abilities for hematologic malignancies are significantly enhanced through immunophenotyping.
Purpose of the Study:
- To delineate specific lineage aberrancies in lymphoid and hematologic neoplasms.
- To describe unique immunophenotypic profiles for various lymphomas and leukemias.
- To explore the diagnostic and prognostic utility of immunohistochemistry (IHC) in these diseases.
Main Methods:
- Immunophenotyping to detect monoclonality (e.g., immunoglobulin restriction).
- Analysis of pan-B or pan-T cell antigen expression and aberrant crosslineage antigen coexpression.
- Immunohistochemistry (IHC) for specific marker analysis (e.g., CD15, CD30, CD45, cyclin D1, CD22, CD11c, CD10).
Main Results:
- Established unique immunophenotypic profiles for most lymphomas and leukemias.
- Identified classic Hodgkin's disease phenotype (CD15+, CD30+, CD45-).
- Described mantle cell lymphoma (cyclin D1+) and hairy cell leukemia (CD22+, CD11c+).
Conclusions:
- Immunophenotyping is crucial for diagnosing lymphoid and hematologic neoplasms.
- Specific immunophenotypic profiles aid in classifying distinct subtypes of lymphoma and leukemia.
- Immunohistochemistry (IHC) offers both diagnostic and prognostic information, impacting treatment strategies.
More Related Videos
Related Concept Videos
01:22Immunocytochemistry and Immunohistochemistry
Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
These...
These...
01:18Lymphoid Cells and Tissues
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...