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Arterial occlusion induces systemic changes in leucocyte composition.

René Haverslag1, Daphne de Groot, Pleunie van den Borne

  • 1Laboratory of Experimental Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands. r.haverslag-3@umcutrecht.nl

European Journal of Clinical Investigation
|February 15, 2011
PubMed
Summary
This summary is machine-generated.

Peripheral arterial occlusion triggers a systemic immune cell shift. Bone marrow changes precede circulating leucocyte fluctuations, indicating an orchestrated response for tissue repair and perfusion recovery.

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Area of Science:

  • Immunology
  • Vascular Biology
  • Hematology

Background:

  • Arterial occlusion causes tissue ischemia, leading to significant mortality and morbidity.
  • Leukocyte extravasation aids tissue repair, but systemic effects of ischemia on leukocyte trafficking are unclear.

Purpose of the Study:

  • To investigate temporal changes in circulating and bone marrow-derived leukocyte fractions after peripheral arterial occlusion in mice.

Main Methods:

  • Femoral artery ligation was performed in mice.
  • Peripheral blood and bone marrow samples were collected at baseline and days 1-7 post-ligation.
  • Leukocyte and bone marrow cell subsets were quantified using flow cytometry.

Main Results:

  • Circulating leukocyte counts remained stable, but significant temporal fluctuations (>50%) were observed in monocytes, lymphocytes, and neutrophils.
  • Granulocytes initially increased, while monocytes and lymphocytes decreased in circulation.
  • Bone marrow progenitor cells (myeloid and lymphoid) upregulated following decreases in their circulating progeny.

Conclusions:

  • Local arterial occlusion induces a coordinated systemic leukocyte trafficking response.
  • This systemic response highlights the critical role of leukocytes in perfusion recovery and tissue remodeling.