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A Sensitive Method to Quantify Senescent Cancer Cells
Published on: August 2, 2013
[The analysis of multi-drug resistance of doxorubicin with flow cytometry]
Guoqiang Zheng1, Xuyi Liu, Fusheng Han
1Department of Surgery, The School of Oncology, Peking University, Beijing 100036, P.R.China.
Background:
To evaluate the significance of flow cytometry (FCM) in the analysis of multi-drug resistance of doxorubicin.
Methods:
The level of doxorubicin or Rh-123 in S-180R and BGC-823/DOX, which were two cell lines with different drug resistance, was measured by FCM comparatively and continuously.
Results:
The total fluorescence profile peaks of S-180R, a high resistant cell line, were predominantly different from those of S-180, the parent cell line, by the FCM analysis. The subtle fluorescence profile differences between BGC-823/DOX (a low resistant cell line) and the parent cell line were quantitatively measured on the FCM map. The changes of each resistant cell fluorescence from the S 180R cells could be displayed by continuous tests, the more near the fluorescence level of each cell was, the more the fluorescence pike of whole cells centralized.
Conclusions:
FCM is a sensitive, accurate and quantitative test in the analysis of doxorubicin drug resistance.
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