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Related Concept Videos

Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: Jun 4, 2026

Single Molecule Fluorescence In Situ Hybridization (smFISH) Analysis in Budding Yeast Vegetative Growth and Meiosis
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[Bioinformatics analysis on CK8 full length coding sequence and eukaryotic expression in SMMC7721 cells].

Meng Xun1, Si-Hai Zhao, Chun-Xia Cao

  • 1Department of Immunology and Microbiology, Xi'an Medical School, Xi'an 710021, China.

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi = Chinese Journal of Cellular and Molecular Immunology
|February 15, 2011
PubMed
Summary

Researchers successfully created an expression vector for human cytokeratin 8 (CK8) and confirmed its overexpression in SMMC7721 cells, paving the way for further CK8 function studies.

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biotechnology

Context:

  • Cytokeratin 8 (CK8) is an important intermediate filament protein involved in various cellular processes.
  • Understanding CK8 expression is crucial for studying its role in normal physiology and disease states.

Purpose:

  • To construct a eukaryotic expression vector encoding human full-length cytokeratin 8 (CK8).
  • To verify the successful expression of the CK8 gene in SMMC7721 cells.

Summary:

  • Human CK8 cDNA was amplified and cloned into the pEGFP-C1 vector, creating pEGFP-CK8.
  • The recombinant plasmid was transfected into SMMC7721 cells, and CK8 expression was confirmed via fluorescence microscopy, real-time PCR, and Western blot.
  • Bioinformatics analysis predicted protein properties, signal peptides, and functional motifs.

Impact:

  • Successfully established a tool for studying CK8's biological functions.
  • Provides a foundation for investigating CK8's role in cellular mechanisms and potential therapeutic targets.