Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 4, 2026

Elevated Plus Maze for Mice
09:09

Elevated Plus Maze for Mice

Published on: December 22, 2008

Increased anxiety-related behaviour in Hint1 knockout mice.

Jeeva Varadarajulu1, Maria Lebar, Gurumoorthy Krishnamoorthy

  • 1Research Group of Proteomics & Biomarkers and Research Group of Psychoneuroendocrinology, Max Planck Institute of Psychiatry, Kraepelinstrasse 2, Munich, Germany.

Behavioural Brain Research
|February 15, 2011
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fecal glucocorticoid metabolites as stress biomarkers in common buzzards (<i>Buteo buteo</i>) across rehabilitation phases: implications for raptor welfare.

Frontiers in veterinary science·2026
Same author

Long-term effects of forty-hertz auditory stimulation as a treatment of Alzheimer's disease: Insights from an aged monkey model study.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Proteomic Signatures of Hippocampal Nonsynaptic and Synaptosome-Enriched Mitochondria in Rats Resilient to Chronic Social Isolation.

Biomolecules·2025
Same author

Prefrontal cortex cytosolic and synaptosome proteome reveal molecular signatures of resilience to chronic social isolation stress in rats.

Reviews in the neurosciences·2025
Same author

Lack of evidence for a consistent differential impact of tail and tunnel handling on markers of welfare in laboratory mice.

Scientific reports·2025
Same author

Prefrontal Cortex Molecular Signatures of Chronically Socially Isolated Rats and Their Response to Fluoxetine Treatment.

Molecular neurobiology·2025

Histidine triad nucleotide-binding protein-1 (Hint1) knockout mice show heightened anxiety and stress responses. This suggests Hint1 and protein kinase C gamma (PKCγ) are key in regulating emotional behavior and coping mechanisms.

Area of Science:

  • Neuroscience
  • Behavioral Science
  • Biochemistry

Background:

  • The histidine triad nucleotide-binding protein-1 (Hint1) has been linked to psychiatric disorders.
  • Understanding the precise role of Hint1 in emotional regulation is crucial.

Purpose of the Study:

  • To investigate the behavioral and biochemical effects of Hint1 deficiency in mice.
  • To explore the relationship between Hint1, protein kinase C gamma (PKCγ), and anxiety-related behaviors.

Main Methods:

  • Behavioral testing of Hint1 knockout (Hint1 KO) mice in anxiety and stress paradigms (elevated plus-maze, dark-light box, forced swim test).
  • Biochemical analysis of brain tissue, including Western blotting for PKCγ expression and PKC enzymatic activity assays.

Main Results:

More Related Videos

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice
14:40

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice

Published on: October 27, 2020

Hyponeophagia: A Measure of Anxiety in the Mouse
05:52

Hyponeophagia: A Measure of Anxiety in the Mouse

Published on: May 17, 2011

Related Experiment Videos

Last Updated: Jun 4, 2026

Elevated Plus Maze for Mice
09:09

Elevated Plus Maze for Mice

Published on: December 22, 2008

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice
14:40

Optogenetic Manipulation of Neuronal Activity to Modulate Behavior in Freely Moving Mice

Published on: October 27, 2020

Hyponeophagia: A Measure of Anxiety in the Mouse
05:52

Hyponeophagia: A Measure of Anxiety in the Mouse

Published on: May 17, 2011

  • Hint1 KO mice displayed increased anxiety-like behaviors and heightened emotional arousal in stress tests.
  • Elevated PKCγ protein expression and increased PKC enzymatic activity were observed in Hint1 KO mice.
  • No significant difference in PKCγ mRNA levels suggests post-transcriptional regulation.

Conclusions:

  • Hint1 plays a significant role in modulating anxiety-related and stress-coping behaviors.
  • The findings highlight a potential link between Hint1, PKCγ, and emotional dysregulation in psychiatric conditions.