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Related Experiment Video

Updated: Jun 4, 2026

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

Keratin films for ocular surface reconstruction.

Stephan Reichl1, Maria Borrelli, Gerd Geerling

  • 1Institut für Pharmazeutische Technologie, Technische Universität Braunschweig, Mendelssohnstr. 1, Braunschweig 38106, Germany.

Biomaterials
|February 15, 2011
PubMed
Summary

Human hair keratin films offer a promising, standardized alternative for ocular surface reconstruction, showing improved transparency and strength compared to amniotic membrane while supporting cell growth.

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Area of Science:

  • Biomaterials Science
  • Ophthalmology
  • Tissue Engineering

Background:

  • Human amniotic membrane (AM) is a common substrate for ocular surface reconstruction but has limitations like reduced transparency and donor variability.
  • Standardized alternatives are needed to overcome the disadvantages of AM in clinical applications.

Purpose of the Study:

  • To develop transparent, stable, and transferable films from human hair keratin.
  • To evaluate these keratin films as a potential substrate for ocular surface reconstruction, supporting cellular adhesion and proliferation.

Main Methods:

  • Keratin extraction and film engineering via a multi-step process including dialysis, drying, and curing.
  • Characterization using SDS-PAGE, SEM, X-ray analysis, swelling, water absorption, tensile strength, and UV/VIS light transmission.

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Ex Vivo Corneal Organ Culture Model for Wound Healing Studies
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Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Published on: February 15, 2019

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Last Updated: Jun 4, 2026

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration
11:42

Combination of Microstereolithography and Electrospinning to Produce Membranes Equipped with Niches for Corneal Regeneration

Published on: September 12, 2014

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies
06:46

Ex Vivo Corneal Organ Culture Model for Wound Healing Studies

Published on: February 15, 2019

  • Assessment of corneal epithelial cell growth, seeding efficiency, and detachment on keratin films versus AM.

    • Main Results:

    • Transparent keratin films composed of nanoparticulate structures were successfully developed.
    • Film properties (transparency, strength) were tunable by altering protein composition, additives, and curing parameters.
    • Keratin films exhibited enhanced light transmission and biomechanical strength compared to AM.
    • Corneal epithelial cell behavior on keratin films was comparable to that on AM.

    Conclusions:

    • Human hair keratin films represent a novel and promising alternative for ocular surface reconstruction.
    • These films offer improved characteristics over traditional amniotic membrane grafts.
    • Further development could lead to standardized, high-performance biomaterials for ophthalmic applications.