Detection of DNA/Protein Interactions in Dopamine Receptor Genes

ABSTRUCT: In the previous chapter, we discussed practical methods to study transcription control mechanisms for the D(1A) dopamine receptor gene. Since these processes are mediated by a complex set of nuclear regulatory proteins that interact with specific DNA sequences, methods to delineate these interactions are paramount in understanding transcription control. In the case of the D(1A) gene, we found several consensus binding sites for known transcription factors on the 5'-noncoding region and examined their binding to the nuclear proteins and known transcription factors (1).