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Related Experiment Video

Updated: Jun 4, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Comparative genomic hybridization.

¹Department of Urology, Stanford University School of Medicine, Stanford, CA.

Methods in Molecular Medicine

|February 15, 2011

Summary

Comparative genomic hybridization (CGH) offers a genome-wide analysis of tumor DNA copy number. This technique uses differentially labeled DNA from tumor and normal cells hybridized to metaphase spreads to detect genetic imbalances.

Area of Science:

Genomics
Molecular Biology
Cancer Research

Background:

Genetic imbalances, such as copy number variations, are hallmarks of cancer.
Accurate detection of these alterations is crucial for diagnosis and understanding tumor development.

Purpose of the Study:

To describe the principle and application of Comparative Genomic Hybridization (CGH).
To highlight CGH's utility in surveying genome-wide copy number alterations in tumor DNA.

Main Methods:

Hybridizing differentially labeled tumor DNA (Test DNA) and normal DNA (Reference DNA) to normal metaphase chromosomes.
Utilizing distinct fluorochrome conjugates for labeling Test and Reference DNA.
Analyzing the ratio of fluorescence signals to determine relative DNA copy number across the genome.

More Related Videos

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Related Experiment Videos

Last Updated: Jun 4, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

Published on: February 21, 2015

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

Published on: November 8, 2017

Main Results:

CGH enables a comprehensive, genome-wide assessment of DNA copy number changes.
The method allows for the detection of both amplifications and deletions in tumor cells.
Genetic imbalances in abnormal cells can be visualized and quantified.

Conclusions:

Comparative Genomic Hybridization is a powerful tool for genome-wide copy number analysis.
CGH facilitates the identification of genetic aberrations in cancer.
This technique aids in the characterization of tumor genomes.